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Literature summary for 3.8.1.2 extracted from

  • Pang, B.C.M.; Tsang, J.S.H.
    Mutagenic analysis of the conserved residues in dehalogenase IVa of Burkholderia cepacia MBA4 (2001), FEMS Microbiol. Lett., 204, 135-140.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D11E totally inactive in catalysis Burkholderia cepacia
D11N totally inactive in catalysis Burkholderia cepacia
D11S totally inactive in catalysis Burkholderia cepacia
D181N totally inactive in catalysis Burkholderia cepacia
additional information point mutations on 29 conserved charged and/or polar residues are generated and characterized Burkholderia cepacia
N178D defective in catalysis Burkholderia cepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2-haloalkanoic acid + H2O Burkholderia cepacia
-
D-2-hydroxyalkanoic acid + halide
-
?
L-2-haloalkanoic acid + H2O Burkholderia cepacia MBA4
-
D-2-hydroxyalkanoic acid + halide
-
?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
MBA4
-
Burkholderia cepacia MBA4
-
MBA4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-haloacid + H2O
-
Burkholderia cepacia (R)-2-hydroxyacid + halide
-
?
(S)-2-haloacid + H2O
-
Burkholderia cepacia MBA4 (R)-2-hydroxyacid + halide
-
?
L-2-haloalkanoic acid + H2O
-
Burkholderia cepacia D-2-hydroxyalkanoic acid + halide
-
?
L-2-haloalkanoic acid + H2O
-
Burkholderia cepacia MBA4 D-2-hydroxyalkanoic acid + halide
-
?
monochloroacetate + H2O
-
Burkholderia cepacia glycolate + HCl
-
?
monochloroacetate + H2O
-
Burkholderia cepacia MBA4 glycolate + HCl
-
?