Application | Comment | Organism |
---|---|---|
synthesis | His-tagged L-2-haloacid dehalogenase subtype immobilized for production of D-lactate and D-chloropropionic acid | Pseudomonas sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | covalently immobilized L-2-haloacid dehalogenase subtype shows KM-value of 23.3 mM | Pseudomonas sp. | |
7.5 | - |
2-monochloropropionic acid | pH 8.5, 37°C | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloropropionate + H2O | Pseudomonas sp. | - |
lactate + HCl | - |
? | |
2-chloropropionate + H2O | Pseudomonas sp. CBS3 | - |
lactate + HCl | - |
? | |
L-2-chloropropionic acid + H2O | Pseudomonas sp. | - |
D-2-hydroxypropionic acid + HCl | - |
? | |
L-2-chloropropionic acid + H2O | Pseudomonas sp. CBS3 | - |
D-2-hydroxypropionic acid + HCl | - |
? | |
L-2-haloalkanoic acid + H2O | Pseudomonas sp. | - |
D-2-hydroxyalkanoic acid + halide | - |
? | |
L-2-haloalkanoic acid + H2O | Pseudomonas sp. CBS3 | - |
D-2-hydroxyalkanoic acid + halide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. CBS3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-haloacid + H2O | - |
Pseudomonas sp. | (R)-2-hydroxyacid + halide | - |
? | |
(S)-2-haloacid + H2O | - |
Pseudomonas sp. CBS3 | (R)-2-hydroxyacid + halide | - |
? | |
2-chloropropionate + H2O | - |
Pseudomonas sp. | lactate + HCl | - |
? | |
2-chloropropionate + H2O | - |
Pseudomonas sp. CBS3 | lactate + HCl | - |
? | |
L-2-chloropropionic acid + H2O | - |
Pseudomonas sp. | D-2-hydroxypropionic acid + HCl | - |
? | |
L-2-chloropropionic acid + H2O | - |
Pseudomonas sp. CBS3 | D-2-hydroxypropionic acid + HCl | - |
? | |
L-2-haloalkanoic acid + H2O | - |
Pseudomonas sp. | D-2-hydroxyalkanoic acid + halide | - |
? | |
L-2-haloalkanoic acid + H2O | - |
Pseudomonas sp. CBS3 | D-2-hydroxyalkanoic acid + halide | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme covalently immobilized is stable between 30°C and 85°C | Pseudomonas sp. |
25 | 70 | - |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 11 | - |
Pseudomonas sp. |