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Literature summary for 3.8.1.2 extracted from

  • Diez, A.; Prieto, M.I.; Alvarez, M.J.; Bautista, J.M.; Puyet, A.; Garrido-Pertierra, A.
    Purification and properties of a high-affinity L-2-haloacid dehalogenase from Azotobacter sp. strain RC26 (1996), Lett. Appl. Microbiol., 23, 279-282.
No PubMed abstract available
Search for more literature for 3.8.1.2

Application

Application Comment Organism
synthesis production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry Azotobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Azotobacter sp.
FeSO4 above 10 mM Azotobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 Monochloroacetate pH 8.0, 30°C Azotobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 gel filtration Azotobacter sp.
29000
-
 1 * 29000 SDS-PAGE Azotobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-chloropropionate + H2O Azotobacter sp.
-
 lactate + HCl
-
 ?
2-chloropropionate + H2O Azotobacter sp. RC26
-
 lactate + HCl
-
 ?
L-2-haloalkanoic acid + H2O Azotobacter sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
L-2-haloalkanoic acid + H2O Azotobacter sp. RC26
-
 D-2-hydroxyalkanoic acid + halide
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 100% activity at 10 mM Azotobacter sp.
chloroform 100% activity at 10 mM Azotobacter sp.

Organism

Organism UniProt Comment Textmining
Azotobacter sp.
-
-
-
Azotobacter sp. RC26
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Azotobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
 coupled assay with lactate dehydrogenase Azotobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-haloacid + H2O
-
 Azotobacter sp. (R)-2-hydroxyacid + halide
-
 ?
(S)-2-haloacid + H2O
-
 Azotobacter sp. RC26 (R)-2-hydroxyacid + halide
-
 ?
2-chloropropionate + H2O
-
 Azotobacter sp. lactate + HCl
-
 ?
2-chloropropionate + H2O
-
 Azotobacter sp. RC26 lactate + HCl
-
 ?
L-2-haloalkanoic acid + H2O
-
 Azotobacter sp. D-2-hydroxyalkanoic acid + halide
-
 ?
L-2-haloalkanoic acid + H2O
-
 Azotobacter sp. RC26 D-2-hydroxyalkanoic acid + halide
-
 ?
monochloroacetate + H2O
-
 Azotobacter sp. glycolate + HCl
-
 ?
monochloroacetate + H2O
-
 Azotobacter sp. RC26 glycolate + HCl
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29000 SDS-PAGE Azotobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Azotobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60
-
 Azotobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 activity after 30 min reduced by 50% Azotobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Azotobacter sp.

pI Value

Organism Comment pI Value Maximum pI Value
Azotobacter sp. isoelectric focusing
-
 5.5