Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.8.1.2 extracted from

  • Kurihara, T.; Esaki, N.; Soda, K.
    Bacterial 2-haloacid dehalogenases: structures and reaction mechanisms (2000), J. Mol. Catal. B, 10, 57-65.
No PubMed abstract available
Search for more literature for 3.8.1.2

Application

Application Comment Organism
synthesis L-2-haloacid dehalogenase subtype produces D-2-hydroxyalkanoic acids for chiral synthesis in industry in water and organic solvents Pseudomonas sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pseudomonas sp.
alpha/beta structure Pseudomonas sp.
mutant S175A Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
additional information diverse mutant types with higher KM-values and lower specific activity Pseudomonas sp.
S118A mutant with lower specific activity and higher KM-value Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2-haloalkanoic acid + H2O Pseudomonas sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
monochloroacetate + H2O Pseudomonas sp.
-
 glycolate + HCl
-
 ?
monochloroacetate + H2O Pseudomonas sp. 113
-
 glycolate + HCl
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO enzyme active in 3 M DMSO at 30°C for 5 min Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide mechanism Pseudomonas sp.
a
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product Pseudomonas sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-haloacid + H2O
-
 Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
2-bromohexadecanoate + H2O
-
 Pseudomonas sp. D-2-hydroxyhexadecanoate + HBr
-
 ?
L-2-chloropropionic acid + H2O
-
 Pseudomonas sp. D-2-hydroxypropionic acid + HCl
-
 ?
L-2-haloalkanoic acid + H2O
-
 Pseudomonas sp. D-2-hydroxyalkanoic acid + halide
-
 ?
monobromoacetate + H2O
-
 Pseudomonas sp. glycolate + HBr
-
 ?
monobromoacetate + H2O
-
 Pseudomonas sp. 113 glycolate + HBr
-
 ?
monochloroacetate + H2O
-
 Pseudomonas sp. glycolate + HCl
-
 ?
monochloroacetate + H2O
-
 Pseudomonas sp. 113 glycolate + HCl
-
 ?
monoiodoacetate + H2O
-
 Pseudomonas sp. glycolate + HI
-
 ?
monoiodoacetate + H2O
-
 Pseudomonas sp. 113 glycolate + HI
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 enzyme retains its full activity for 30 min Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Pseudomonas sp.