Crystallization (Comment) | Organism |
---|---|
mutant S114A in complex with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid, 8-(2-chlorophenyl)-2-hydroxy-5-methyl-6-oxoocta-2,4-dienoic acid, or 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid, sitting drop vapor diffusion, 0.001 ml of 8 mg/ml protein solution is mixed with 0.001 ml of precipitant solution containing 200 mM KSCN, 24% PEG 3350, and 100 mM bis-tris propane, pH 7.0, soaking of S114A crystals in 0.01 ml of precipitant solution supplemented with 15 mM of each ligand for 30-60 min, X-ray diffractin structure determination and analysis at 1.8-2.1 A resolution, molecular replacement | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
S114A | active site HsaD mutant, catalytically impaired and binds 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid with an altered dissociation constant compared to the wild-type enzyme | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | pH 7.5, 25°C | Mycobacterium tuberculosis | |
0.31 | - |
8-(2-chlorophenyl)-2-hydroxy-5-methyl-6-oxoocta-2,4-dienoic acid | pH 7.5, 25°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNH5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WNH5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O | i.e. HOPDA, a biphenyl meta-cleavage product | Mycobacterium tuberculosis | 2-hydroxypenta-2,4-dienoic acid + benzoate | - |
? | |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O | i.e. HOPDA, a biphenyl meta-cleavage product | Mycobacterium tuberculosis H37Rv | 2-hydroxypenta-2,4-dienoic acid + benzoate | - |
? | |
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O | i.e. DSHA, a cholesterol meta-cleavage product | Mycobacterium tuberculosis | 3-[(7aR)-7a-hydroxy-1,5-dioxooctahydro-1H-inden-4-yl]propanoic acid + (2Z,4Z)-5-hydroxyhexa-2,4-dienoic acid | - |
? | |
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O | i.e. DSHA, a cholesterol meta-cleavage product | Mycobacterium tuberculosis H37Rv | 3-[(7aR)-7a-hydroxy-1,5-dioxooctahydro-1H-inden-4-yl]propanoic acid + (2Z,4Z)-5-hydroxyhexa-2,4-dienoic acid | - |
? | |
8-(2-chlorophenyl)-2-hydroxy-5-methyl-6-oxoocta-2,4-dienoic acid + H2O | i.e. HOPODA, a HOPDA synthetic analogue | Mycobacterium tuberculosis | 2-hydroxypenta-2,4-dienoic acid + benzoate | - |
? | |
8-(2-chlorophenyl)-2-hydroxy-5-methyl-6-oxoocta-2,4-dienoic acid + H2O | i.e. HOPODA, a HOPDA synthetic analogue | Mycobacterium tuberculosis H37Rv | 2-hydroxypenta-2,4-dienoic acid + benzoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase | - |
Mycobacterium tuberculosis |
HsaD | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | pH 7.5, 25°C | Mycobacterium tuberculosis | |
0.33 | - |
8-(2-chlorophenyl)-2-hydroxy-5-methyl-6-oxoocta-2,4-dienoic acid | pH 7.5, 25°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the intracellular survival of pathogen Mycobacterium tuberculosis | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
8-(2-chlorophenyl)-2-hydroxy-5-methyl-6-oxoocta-2,4-dienoic acid | pH 7.5, 25°C | Mycobacterium tuberculosis | |
9 | - |
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | pH 7.5, 25°C | Mycobacterium tuberculosis |