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Literature summary for 3.7.1.8 extracted from

  • Seah, S.Y.; Labbe, G.; Nerdinger, S.; Johnson, M.R.; Snieckus, V.; Eltis, L.D.
    Identification of a serine hydrolase as a key determinant in the microbial degradation of polychlorinated biphenyls (2000), J. Biol. Chem., 275, 15701-15708.
    View publication on PubMed
Search for more literature for 3.7.1.8

Inhibitors

Inhibitors Comment Organism Structure
(2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid competitively inhibits Burkholderia cepacia
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid competitively inhibits Burkholderia cepacia
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid competitively inhibits Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00013
-
 (2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
 Burkholderia cepacia
0.00019
-
 (2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid not chlorinated Burkholderia cepacia
0.00033
-
 (2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Burkholderia cepacia
0.00046
-
 (2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Burkholderia cepacia
0.00054
-
 (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Burkholderia cepacia
0.00095
-
 (2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Burkholderia cepacia
0.0036
-
 (2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Burkholderia cepacia
0.0049
-
 (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-
Burkholderia cepacia LB400
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to apparent homogeneity Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
 Burkholderia cepacia 4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
 Burkholderia cepacia LB400 4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Burkholderia cepacia benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Burkholderia cepacia LB400 benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Burkholderia cepacia 2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Burkholderia cepacia LB400 2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Burkholderia cepacia 3-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Burkholderia cepacia LB400 3-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Burkholderia cepacia benzoate + (2E)-2,4-dihydroxypenta-2,4-dienoic acid
-
 ?
(2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Burkholderia cepacia LB400 benzoate + (2E)-2,4-dihydroxypenta-2,4-dienoic acid
-
 ?
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Burkholderia cepacia benzoate + (2E)-4-chloro-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Burkholderia cepacia benzoate + (2E,4Z)-5-chloro-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Burkholderia cepacia benzoate + (2Z)-3-chloro-2-hydroxypenta-2,4-dienoic acid
-
 ?

Synonyms

Synonyms Comment Organism
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
-
 Burkholderia cepacia
BphD
-
 Burkholderia cepacia
HOPDA hydrolase
-
 Burkholderia cepacia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00059
-
 (2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Burkholderia cepacia
0.0055
-
 (2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Burkholderia cepacia
0.0089
-
 (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Burkholderia cepacia
1.53
-
 (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Burkholderia cepacia
2.1
-
 (2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Burkholderia cepacia
3.13
-
 (2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
 Burkholderia cepacia
3.9
-
 (2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Burkholderia cepacia
4.18
-
 (2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid not chlorinated Burkholderia cepacia

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00057
-
 (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Burkholderia cepacia
0.00095
-
 (2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Burkholderia cepacia
0.0036
-
 (2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Burkholderia cepacia