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Literature summary for 3.7.1.8 extracted from

  • Seah, S.Y.; Labbe, G.; Kaschabek, S.R.; Reifenrath, F.; Reineke, W.; Eltis, L.D.
    Comparative specificities of two evolutionarily divergent hydrolases involved in microbial degradation of polychlorinated biphenyls (2001), J. Bacteriol., 183, 1511-1516.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.7.1.8

Application

Application Comment Organism
degradation key determinant in the aerobic transformation of polychlorinated biphenyls by divergent biphenyl degraders Rhodococcus globerulus

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Pseudomonas putida KT2442 containing vector pSS316 Rhodococcus globerulus

Inhibitors

Inhibitors Comment Organism Structure
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid competetively inhibits the enzyme more effectively than (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Rhodococcus globerulus
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00043
-
 (2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
0.00047
-
 (2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid not chlorinated Rhodococcus globerulus
0.00065
-
 (2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
0.00075
-
 (2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
0.00104
-
 (2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
0.004
-
 (2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus
0.0044
-
 (2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus
0.0069
-
 (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus
0.0129
-
 (2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
0.018
-
 (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32580
-
 by HPLC-ESMS analysis Rhodococcus globerulus

Organism

Organism UniProt Comment Textmining
Rhodococcus globerulus
-
-
-
Rhodococcus globerulus P6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to more than 99% homogeneity Rhodococcus globerulus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.2
-
 purified enzyme Rhodococcus globerulus

Storage Stability

Storage Stability Organism
-80°C, 12 months Rhodococcus globerulus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus 4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus P6 4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus P6 benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus 2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus P6 2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus 3,4-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus P6 3,4-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus 3,5-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus P6 3,5-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus 3-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus benzoate + (2E)-2,4-dihydroxypenta-2,4-dienoic acid
-
 ?
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus benzoate + (2E)-4-chloro-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus benzoate + (2E,4Z)-5-chloro-2-hydroxypenta-2,4-dienoic acid
-
 ?
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus benzoate + (2Z)-3-chloro-2-hydroxypenta-2,4-dienoic acid
-
 ?

Synonyms

Synonyms Comment Organism
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
-
 Rhodococcus globerulus
BphDP6
-
 Rhodococcus globerulus
HOPDA hydrolase
-
 Rhodococcus globerulus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0172
-
 (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus
1.03
-
 (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus
1.74
-
 (2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
7.6
-
 (2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid not chlorinated Rhodococcus globerulus
11.2
-
 (2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
15.3
-
 (2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
29
-
 (2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
33.7
-
 (2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus