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Literature summary for 3.7.1.8 extracted from

  • Buenz, P.V.; Falchetto, R.; Cook, A.M.
    Purification of two isofunctional hydrolases (EC 3.7.1.8) in the degradative pathway for dibenzofuran in Sphingomonas sp. strain RW1 (1993), Biodegradation, 4, 171-178.
    View publication on PubMed
Search for more literature for 3.7.1.8

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 1 * 29000, isoenzyme H2, SDS-PAGE Sphingomonas sp.
31000
-
 1 * 31000, isoenzyme H1, SDS-PAGE Sphingomonas sp.
37000 40000 isoenzyme H1, gel filtration, non-denaturing PAGE Sphingomonas sp.
41000 48000 isoenzyme H2, gel filtration, non-denaturing PAGE Sphingomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sphingomonas sp. enzyme is involved in the degradative pathway for dibenzofuran ?
-
 ?
additional information Sphingomonas sp. RW1 enzyme is involved in the degradative pathway for dibenzofuran ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingomonas sp.
-
 2 isofunctional hydrolases H1 and H2
-
Sphingomonas sp. RW1
-
 2 isofunctional hydrolases H1 and H2
-

Purification (Commentary)

Purification (Comment) Organism
2 isofunctional hydrolases H1 and H2 Sphingomonas sp.

Storage Stability

Storage Stability Organism
-20°C or -70°C, buffer containing 30% glycerol, stable for 1 week Sphingomonas sp.
4°C, 48 h, 10% loss of isoenzyme H1 activity, 20% loss of isoenzyme H2 activity Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)hexa-2,4-dienoate + H2O
-
 Sphingomonas sp. 2-oxopent-4-enoate + salicylate
-
 ?
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)hexa-2,4-dienoate + H2O
-
 Sphingomonas sp. RW1 2-oxopent-4-enoate + salicylate
-
 ?
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Sphingomonas sp. benzoate + 2-oxopent-4-enoate
-
 ?
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Sphingomonas sp. RW1 benzoate + 2-oxopent-4-enoate
-
 ?
additional information enzyme is involved in the degradative pathway for dibenzofuran Sphingomonas sp. ?
-
 ?
additional information enzyme is involved in the degradative pathway for dibenzofuran Sphingomonas sp. RW1 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 31000, isoenzyme H1, SDS-PAGE Sphingomonas sp.
monomer 1 * 29000, isoenzyme H2, SDS-PAGE Sphingomonas sp.