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Literature summary for 3.6.5.4 extracted from
- Concerted complex assembly and GTPase activation in the chloroplast signal recognition particle (2011), Biochemistry, 50, 7208-7217.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant cpSRP54 in Escherichia coli strain Rosetta BL21 | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A142W | site-directed mutagenesis, the mutant cpSRP54 exhibits the same GTP-dependent complex assembly kinetics as wild-type cpSRP54 | Arabidopsis thaliana |
| A143L | site-directed mutagenesis, mutant cpSRP54 GTP-dependent complex assembly kinetics compared to the wild-type cpSRP54, overview | Arabidopsis thaliana |
| A143W | site-directed mutagenesis, mutant cpSRP54 GTP-dependent complex assembly kinetics compared to the wild-type cpSRP54, overview | Arabidopsis thaliana |
| A168W | site-directed mutagenesis, mutant cpSRP54 GTP-dependent complex assembly kinetics compared to the wild-type cpSRP54, overview | Arabidopsis thaliana |
| A169L | site-directed mutagenesis, mutant cpSRP54 GTP-dependent complex assembly kinetics compared to the wild-type cpSRP54, overview | Arabidopsis thaliana |
| A169W | site-directed mutagenesis, mutant cpSRP54 GTP-dependent complex assembly kinetics compared to the wild-type cpSRP54, overview | Arabidopsis thaliana |
| D137A | site-directed mutagenesis, mutant cpSRP54 GTP-dependent complex assembly kinetics compared to the wild-type cpSRP54, overview | Arabidopsis thaliana |
| D163A | site-directed mutagenesis, mutant cpSRP54 GTP-dependent complex assembly kinetics compared to the wild-type cpSRP54, overview | Arabidopsis thaliana |
| additional information | binding kinetics and competing inhibitions with several bacterial cpFtsY mutants, affinities of mutant bacterial cpFtsYs for cpSRP54, and complex formations, overview | Arabidopsis thaliana |
| R140A | site-directed mutagenesis, mutant cpSRP54 GTP-dependent complex assembly kinetics compared to the wild-type cpSRP54, overview | Arabidopsis thaliana |
| R166A | site-directed mutagenesis, mutant cpSRP54 GTP-dependent complex assembly kinetics compared to the wild-type cpSRP54, overview | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | chloroplast SRP | Arabidopsis thaliana | 9507 | - |
| cytosol | cytosolic SRP | Arabidopsis thaliana | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Arabidopsis thaliana | - |
GDP + phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
gene cpSRP54 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant cpSRP54 from Escherichia coli strain Rosetta BL21 by two steps of cation exchange chromatography | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Arabidopsis thaliana | GDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | homology modeling and structure analysis, overview | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cpSRP54 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Arabidopsis thaliana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | concerted complex assembly and GTPase activation occurs in the chloroplast signal recognition particle. In contrast to the cytosolic homologues, GTPase activation in the chloroplast SRP-SR complex contributes marginally to the targeting of LHC proteins. Complex assembly and GTPase activation are highly coupled in the chloroplast SRP and SR and suggest that the chloroplast GTPases may forego the GTPase activation step as a key regulatory point. Homology model of the cpSRP54·cpFtsY complex based on superposition of the crystal structure of apo-cpFtsY onto that of Thermus aquaticus SR, i.e. FtsY, in complex with Ffh. Thermodynamic and kinetics for formation of the cpSRP54-cpFtsY complex, formed by wild-types and mutants, detailed overview. IBD loops play essential roles in both complex assembly and GTPase activation | Arabidopsis thaliana |
| physiological function | the universally conserved signal recognition particle, SRP, and SRP receptor, SR, mediate the cotranslational targeting of proteins to cellular membranes. In contrast, a unique chloroplast SRP in green plants is primarily dedicated to the post-translational targeting of light harvesting chlorophyll a/b binding proteins. In both pathways, dimerization and activation between the SRP and SR GTPases mediate the delivery of cargo. Efficient assembly of the cpSRP54-cpFtsY complex is crucial for the targeting and integration of LHCP, whereas GTPase activation and/or GTP hydrolysis plays a modulatory role to help enhance the efficiency of targeting | Arabidopsis thaliana |
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