Crystallization (Comment) | Organism |
---|---|
crystal structure analysis of enzyme complexed with the GTP analogue GDPNP and with GDP at 2.0 A resolution , PDB IDs 1G7T and 1G7S, enzyme in complex with GTP/Mg2+, modeling and molecular dynamics simulations | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamics of nucleotide binding | Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Methanothermobacter thermautotrophicus | - |
GDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + H2O = GDP + phosphate | catalytic mechanism, structure-function relationship, overview | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Methanothermobacter thermautotrophicus | GDP + phosphate | - |
? | |
GTP + H2O | two models of the reaction mechanism using the crystal structure: I. Glu81 becomes protonated upon GTP binding, with preference to bind GDP apparently contradicting its assignment as ON, or II. Glu81 protonation/deprotonation defines the ON/OFF states. Protonated Glu81, is ON, whereas X-ray(GTP):GDP is OFF. The model postulates that distant conformational changes such as domain IV rotation are uncoupled from GTP/GDP exchange and do not affect the relative GTP/GDP binding affinities. Glu81-GTP interaction helps to hold switch 2 in place, if Glu81 is deprotonated, it and nearby residues move away from their crystal positions | Methanothermobacter thermautotrophicus | GDP + phosphate | - |
? | |
additional information | GTP/GDP binding analysis using molecular dynamics and a continuum electrostatic free energy method | Methanothermobacter thermautotrophicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aIF5B | - |
Methanothermobacter thermautotrophicus |
GTPase aIF5B | - |
Methanothermobacter thermautotrophicus |
initiation factor aIF5B | - |
Methanothermobacter thermautotrophicus |
General Information | Comment | Organism |
---|---|---|
physiological function | the GTPase aIF5B is a universally conserved initiation factor that assists ribosome assembly | Methanothermobacter thermautotrophicus |