Literature summary for 3.6.5.3 extracted from

Blombach, F.; Launay, H.; Zorraquino, V.; Swarts, D.C.; Cabrita, L.D.; Benelli, D.; Christodoulou, J.; Londei, P.; van der Oost, J.
An HflX-type GTPase from Sulfolobus solfataricus binds to the 50S ribosomal subunit in all nucleotide-bound states (2011), J. Bacteriol., 193, 2861-2867.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0053	-	GTP	pH 7.4, 50°C, in absence of ribosomes	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q980M3	-	-
Saccharolobus solfataricus P2	Q980M3	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	ATP hydrolysis is insignificant compared to the levels of GTP hydrolysis	Saccharolobus solfataricus	GDP + phosphate	-	?
GTP + H2O	ATP hydrolysis is insignificant compared to the levels of GTP hydrolysis	Saccharolobus solfataricus P2	GDP + phosphate	-	?

Subunits

Subunits	Comment	Organism
More	the enzyme binds to the 50S ribosomal subunit	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
HflX GTPase	-	Saccharolobus solfataricus
SSO0269	locus name	Saccharolobus solfataricus
SsoHflX	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	assay at	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00092	-	GTP	pH 7.4, 50°C, in absence of ribosomes	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.17	-	GTP	pH 7.4, 50°C, in absence of ribosomes	Saccharolobus solfataricus

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Release 2023.1 (January 2023)