Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
D60A | 1.4fold slower hydrolysis of GTP | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
GTP | mutant D60A | Saccharolobus solfataricus | |
0.0027 | - |
GTP | - |
Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
gel filtration | Saccharolobus solfataricus |
49000 | - |
SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | 60°C | Saccharolobus solfataricus | GDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1*49000, SDS-PAGE and gel filtration | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
EF 1 alpha | - |
Saccharolobus solfataricus |
elongation factor 1 alpha | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
87 | 96 | half-inactivation time 99 min at 87°C, half-inactivation time 3.5 min at 96°C | Saccharolobus solfataricus |
93.6 | - |
temperature for half denaturation of D60A mutant | Saccharolobus solfataricus |
95.1 | - |
temperature for half denaturation | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
GDP | - |
Saccharolobus solfataricus | |
0.0029 | - |
GDP | mutant D60A | Saccharolobus solfataricus |