Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | competitive with GTP | Saccharolobus solfataricus | |
guanyl-5'-yl imidodiphosphate | competitive with GTP | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
GTP | pH 7.8, 50°C | Saccharolobus solfataricus | |
0.009 | - |
GTP | pH 7.8, 60°C | Saccharolobus solfataricus | |
0.01 | - |
GTP | pH 7.8, 70°C | Saccharolobus solfataricus | |
0.011 | - |
GTP | pH 7.8, 75°C | Saccharolobus solfataricus | |
0.013 | - |
GTP | pH 7.8, 80°C | Saccharolobus solfataricus | |
0.032 | - |
GTP | pH 7.8, 87°C | Saccharolobus solfataricus | |
0.148 | - |
GTP | pH 7.8, 91°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
elongation factor 2 exhibits an intrinsic hardly detectable GTPase activity that is stimulated by ribosomes up to 2000-fold | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Saccharolobus solfataricus | GDP + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
both Km for GTP and kcat of GTPaser increase with increasing temperature | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
GTP | pH 7.8, 50°C | Saccharolobus solfataricus | |
0.8 | - |
GTP | pH 7.8, 60°C | Saccharolobus solfataricus | |
1.7 | - |
GTP | pH 7.8, 70°C | Saccharolobus solfataricus | |
2.7 | - |
GTP | pH 7.8, 75°C | Saccharolobus solfataricus | |
3.5 | - |
GTP | pH 7.8, 80°C | Saccharolobus solfataricus | |
6.2 | - |
GTP | pH 7.8, 87°C | Saccharolobus solfataricus | |
8.8 | - |
GTP | pH 7.8, 91°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Saccharolobus solfataricus |