Cloned (Comment) | Organism |
---|---|
comparison of sequence with that of Sulfolobus solfataricus strain MT4 shows only one amino acid change, i.e. I15V. The difference is in the first guanine nucleotide binding consensus sequence G13HIDHGK and is responsible for a increased efficiency in protein synthesis, which is accompanied by an reduced affinity for both guanosine diphosphate (GDP) and guanosine triphosphate (GTP), and an decreased efficiency in the intrinsic GTPase activity | Saccharolobus solfataricus |
comparison of sequence with that of Sulfolobus solfataricus strain MT4 shows only one amino acid change, i.e. V15I. The difference is in the first guanine nucleotide binding consensus sequence G13HIDHGK and is responsible for a reduced efficiency in protein synthesis, which is accompanied by an increased affinity for both guanosine diphosphate (GDP) and guanosine triphosphate (GTP), and an increased efficiency in the intrinsic GTPase activity | Saccharolobus solfataricus |
the gene encoding SsEF-1alpha from the strain MT3 is cloned, sequenced and expressed in Escherichia coli, the vectors pGEM-3Z and pET22 are used | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | comparison of sequence with that of Sulfolobus solfataricus strain MT4 shows only one amino acid change, i.e. I15V. The difference is in the first guanine nucleotide binding consensus sequence G13HIDHGK and is responsible for a increased efficiency in protein synthesis, which is accompanied by an reduced affinity for both guanosine diphosphate (GDP) and guanosine triphosphate (GTP), and an decreased efficiency in the intrinsic GTPase activity. The exchange has only very marginal effects on the thermal properties of the enzyme | Saccharolobus solfataricus |
additional information | comparison of sequence with that of Sulfolobus solfataricus strain MT4 shows only one amino acid change, i.e. V15I. The difference is in the first guanine nucleotide binding consensus sequence G13HIDHGK and is responsible for a reduced efficiency in protein synthesis, which is accompanied by an increased affinity for both guanosine diphosphate (GDP) and guanosine triphosphate (GTP), and an increased efficiency in the intrinsic GTPase activity. The exchange has only very marginal effects on the thermal properties of the enzyme | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
GTP | SsMT4EF-1alpha | Saccharolobus solfataricus | |
0.0027 | - |
GTP | SsMT3EF-1alpha | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
ribosome | - |
Saccharolobus solfataricus | 5840 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Saccharolobus solfataricus | - |
GDP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
elongation factor 1alpha from strain MT3 with optimum growth temperature 75°C | - |
Saccharolobus solfataricus | - |
elongation factor 1alpha from strain MT4 with optimum growth temperature 87°C | - |
Saccharolobus solfataricus | P35021 | strain MT3, optimal growth temperature 75°C, strain MT4, optimal growth temperature 87°C | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Saccharolobus solfataricus | GDP + phosphate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
EF-1alpha | - |
Saccharolobus solfataricus |
elongation factor 1alpha | - |
Saccharolobus solfataricus |
protein-sythesizing GTPase | - |
Saccharolobus solfataricus |
SsEF-1alpha | amino acid at position 15, strain MT3 valine, SsMT3EF-1alpha, strain MT4 isoleucine, SsMT4EF-1alpha | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
87 | - |
SsMT3EF-1alpha | Saccharolobus solfataricus |
91 | - |
SsMT4EF-1alpha | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
GTP | SsMT3EF-1alpha | Saccharolobus solfataricus | |
0.015 | - |
GTP | SsMT4EF-1alpha | Saccharolobus solfataricus |