Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.5.3 extracted from

  • Masullo, M.; Ianniciello, G.; Arcari, P.; Bocchini, V.
    Properties of truncated forms of the elongation factor 1alpha from the archaeon Sulfolobus solfataricus (1997), Eur. J. Biochem., 243, 468-473.
    View publication on PubMed
Search for more literature for 3.6.5.3

Activating Compound

Activating Compound Comment Organism Structure
NaCl
-
 Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus
the SsEF-1alpha gene is cloned into the pT7-7 vector for expression in Escherichia coli BL21DE3 cells, in addition two truncated forms of SsEF-1alpha are constructed, encoding the domain G and the domains G+M Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
additional information construction of truncated forms corresponding to the putative domains G+M, and domain G. Neither truncated form is able to sustain poly(Phe) synthesis but they are able to bind guanine nucleotides with an affinity much higher with respect to that of the intact factor. Kinetic data are not changed by the truncation, but both forms are less thermostable than the intact factor and both are no more sensitive to the stimulatory effect of elongation factor 1beta Saccharolobus solfataricus
Ss(G)EF-1alpha truncated form of SsEF-1alpha Saccharolobus solfataricus
Ss(GM)EF-1alpha truncated form of SsEF-1alpha Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
GDP
-
 Saccharolobus solfataricus
guanosine 5'-(beta,gamma-imido)triphosphate
-
 Saccharolobus solfataricus
guanyl-5'-yl imidotriphosphate
-
 Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 GTP truncated mutant, domains G+M, 60°C, pH 7.8 Saccharolobus solfataricus
0.0023
-
 GTP Ss(GM)EF-1alpha Saccharolobus solfataricus
0.0024
-
 GTP truncated mutant, domain G, 60°C, pH 7.8 Saccharolobus solfataricus
0.0024
-
 GTP Ss(G)EF-1alpha Saccharolobus solfataricus
0.0025
-
 GTP wild-type, 60°C, pH 7.8 Saccharolobus solfataricus
0.0025
-
 GTP SsEF-1alpha Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
ribosome
-
 Saccharolobus solfataricus 5840
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 truncated G-form of SsEF-1alpha, determined by SDS-PAGE and Western blot Saccharolobus solfataricus
38000
-
 truncated GM-form of SsEF-1alpha, determined by SDS-PAGE and Western blot Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Saccharolobus solfataricus
-
 GDP + phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus
-
 elongation factor 1alpha
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
-20°C, Tris/HCl buffer, pH 7.8, 10 mM MgCl2, 50 mM KCl, 50% v/v glycerol Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Saccharolobus solfataricus GDP + phosphate
-
 ?
GTP + H2O
-
 Saccharolobus solfataricus GDP + phosphate
-
 ir

Subunits

Subunits Comment Organism
monomer
-
 Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
elongation factor 1alpha
-
 Saccharolobus solfataricus
protein-sythesizing GTPase
-
 Saccharolobus solfataricus
SsEF-1alpha
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 ATPase assay Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 GTP Ss(G)EF-1alpha Saccharolobus solfataricus
0.01
-
 GTP truncated mutant, domain G, 60°C, pH 7.8 Saccharolobus solfataricus
0.014
-
 GTP wild-type, 60°C, pH 7.8 Saccharolobus solfataricus
0.014
-
 GTP SsEF-1alpha Saccharolobus solfataricus
0.018
-
 GTP truncated mutant, domains G+M, 60°C, pH 7.8 Saccharolobus solfataricus
0.018
-
 GTP Ss(GM)EF-1alpha Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 ATPase assay Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0013
-
 GDP wild-type, 60°C, pH 7.8 Saccharolobus solfataricus
0.0013
-
 GDP SsEF-1alpha Saccharolobus solfataricus
0.0017
-
 GDP truncated mutant, domain G, 60°C, pH 7.8 Saccharolobus solfataricus
0.0017
-
 GDP Ss(G)EF-1alpha Saccharolobus solfataricus
0.0062
-
 GDP truncated mutant, domains G+M, 60°C, pH 7.8 Saccharolobus solfataricus
0.0062
-
 GDP Ss(GM)EF-1alpha Saccharolobus solfataricus
0.0084
-
 guanyl-5'-yl imidotriphosphate wild-type, 60°C, pH 7.8 Saccharolobus solfataricus
0.0084
-
 guanosine 5'-(beta,gamma-imido)triphosphate SsEF-1alpha Saccharolobus solfataricus
0.0193
-
 guanyl-5'-yl imidotriphosphate truncated mutant, domain G, 60°C, pH 7.8 Saccharolobus solfataricus
0.0193
-
 guanosine 5'-(beta,gamma-imido)triphosphate Ss(G)EF-1alpha Saccharolobus solfataricus
0.0407
-
 guanyl-5'-yl imidotriphosphate truncated mutant, domains G+M, 60°C, pH 7.8 Saccharolobus solfataricus
0.0407
-
 guanosine 5'-(beta,gamma-imido)triphosphate Ss(GM)EF-1alpha Saccharolobus solfataricus