Literature summary for 3.6.5.3 extracted from

Tanfani, F.; Scire, A.; Masullo, M.; Raimo, G.; Bertoli, E.; Bocchini, V.
Salts induce structural changes in elongation factor 1alpha from the hyperthermophilic archaeon Sulfolobus solfataricus: a Fourier transform infrared spectroscopic study (2001), Biochemistry, 40, 13143-13148.

Activating Compound

Activating Compound	Comment	Organism	Structure
NaCl	intrinsic GTPase activity of elongation factor 1alpha is triggered in vitro by NaCl at molar concentrations. The sodium ion is responsible for the induction of the GTPase activity, whereas the anion modulates the enzymatic activity through destabilization of particular regions of the protein	Saccharolobus solfataricus
NaCl	intrinsic GTPase activity that is triggered in vitro by NaCl at molar concentrations	Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment)	Organism
for expression in Escherichia coli cells	Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment)	Organism
Fourier transform infrared spectroscopic study. Substitution of the GDP bound with guanyl-5'-yl imido diphosphate induces a slight increase in the alpha helix and beta sheet content. The alpha helix content of the enzyme-GDP complex increases upon addition of salts, and the highest effect is produced by 5 M NaCl. Thermal stability of the enzyme-GDP complex is significantly reduced when the GDP is replaced with guanyl-5'-yl imido diphosphate or in the presence of NaBr or NH4Cl	Saccharolobus solfataricus

Crystallization (Comment)

Organism

Fourier transform infrared spectroscopic study. Substitution of the GDP bound with guanyl-5'-yl imido diphosphate induces a slight increase in the alpha helix and beta sheet content. The alpha helix content of the enzyme-GDP complex increases upon addition of salts, and the highest effect is produced by 5 M NaCl. Thermal stability of the enzyme-GDP complex is significantly reduced when the GDP is replaced with guanyl-5'-yl imido diphosphate or in the presence of NaBr or NH4Cl

Saccharolobus solfataricus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
ribosome	-	Saccharolobus solfataricus	5840	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Saccharolobus solfataricus
Mn2+	-	Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Saccharolobus solfataricus	-	GDP + phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-
Saccharolobus solfataricus	-	elongation factor 1alpha	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Saccharolobus solfataricus	GDP + phosphate	-	ir

Synonyms

Synonyms	Comment	Organism
elongation factor 1alpha	-	Saccharolobus solfataricus
protein-sythesizing GTPase	-	Saccharolobus solfataricus
SsEF-1alpha	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	activity assay	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	thermal stability of the enzyme-GDP complex is significantly reduced when the GDP is replaced with guanyl-5'-yl imido diphosphate or in the presence of NaBr or NH4Cl	Saccharolobus solfataricus