Literature summary for 3.6.5.3 extracted from

Van Noort, J.M.; Kraal, B.; Sinjorgo, K.M.; Persoon, N.L.M.; Johanns, E.S.D.; Bosch, L.
Methylation in vivo of elongation factor EF-Tu at lysine-56 decreases the rate of tRNA-dependent GTP hydrolysis (1986), Eur. J. Biochem., 160, 557-561.

Search for more literature for 3.6.5.3

Application

Application	Comment	Organism
medicine	protein biosynthesis	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Escherichia coli	-	GDP + phosphate	-	?
guanosine 5'-(thio)triphosphate + H2O	Escherichia coli	-	GDP + thiophosphate + 3 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	elongations factor (EF)Tu	-
Escherichia coli	-	strains MRE600, unmethylated enzyme, and LBE 1001, methylated enzyme	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
side-chain modification	methylation of lysine-56, enzyme with decreased rate of tRNA-dependent GTP hydrolysis	Escherichia coli

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Escherichia coli	GDP + phosphate	-	?
guanosine 5'-(thio)triphosphate + H2O	-	Escherichia coli	GDP + thiophosphate + 3 H+	-	?

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Release 2023.1 (January 2023)