Literature summary for 3.6.5.2 extracted from

Kobayashi, T.; Hori, Y.; Ueda, N.; Kajiho, H.; Muraoka, S.; Shima, F.; Kataoka, T.; Kontani, K.; Katada, T.
Biochemical characterization of missense mutations in the Arf/Arl-family small GTPase Arl6 causing Bardet-Biedl syndrome (2009), Biochem. Biophys. Res. Commun., 381, 439-442.

Search for more literature for 3.6.5.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-Codon-Plus(DE3) cells, and in MDCKII and Flp-In-293 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
G169A	the mutation causes the Bardet-Biedl syndrome, the mutant protein is destabilized probably due to the weak affinity to guanine nucleotides	Homo sapiens
L170W	the mutation causes the Bardet-Biedl syndrome, the mutant protein is destabilized probably due to the weak affinity to guanine nucleotides	Homo sapiens
T31M	the mutation causes the Bardet-Biedl syndrome, the mutant protein is destabilized probably due to the weak affinity to guanine nucleotides	Homo sapiens
T31N	the guanine nucleotide-free Arl6 mutant protein is unstable and degraded in living cells	Homo sapiens
T31R	the mutation selectively abrogates the GTP-binding ability of Arl6 without affecting GDP-binding/dissociating properties	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
GTPgammaS	Arl6 is competitively inhibited by the increasing concentrations of non-radioactive GTPgammaS	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione Sepharose 4B column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Homo sapiens	GDP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
Arl6	-	Homo sapiens

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Release 2023.1 (January 2023)