Literature summary for 3.6.5.2 extracted from

Yu, Y.; Chang, Y.; Li, S.; Hu, H.; Huang, Q.; Ding, J.
Expression, purification, crystallization and preliminary structural characterization of the GTPase domain of human Rheb (2004), Acta Crystallogr. Sect. D, D60, 1883-1887.

Cloned(Commentary)

Cloned (Comment)	Organism
GTPase domain of human Rheb recombinantly expressed in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
GTPase domain of human Rheb recombinantly expressed in Escherichia coli, purified and cocrystallized in complexes with GDP, GTP and GppNHp using the hanging-drop vapour-diffusion method. Crystals of the hRheb/GDP complex belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 44.5, b = 52.3, c = 70.6 A. The hRheb/GppNHp complex crystallizes in two crystal forms: one has the same space group and unit-cell parameters as the hRheb/GDP complex and the other belongs to space group C222(1), with unit-cell parameters a = 102.9, b = 99.2, c = 48.0 A. The hRheb/GTP complex also crystallizes in two crystal forms: one belongs to space group C222(1), with unit-cell parameters a = 102.4, b = 98.3, c = 47.9 A, and the other belongs to space group P2(1), with unit-cell parameters a = 77.3, b = 47.9, c = 71.9 A, beta = 89°. All these crystals diffract X-rays to better than 2.8 A resolution	Homo sapiens

Crystallization (Comment)

Organism

GTPase domain of human Rheb recombinantly expressed in Escherichia coli, purified and cocrystallized in complexes with GDP, GTP and GppNHp using the hanging-drop vapour-diffusion method. Crystals of the hRheb/GDP complex belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 44.5, b = 52.3, c = 70.6 A. The hRheb/GppNHp complex crystallizes in two crystal forms: one has the same space group and unit-cell parameters as the hRheb/GDP complex and the other belongs to space group C222(1), with unit-cell parameters a = 102.9, b = 99.2, c = 48.0 A. The hRheb/GTP complex also crystallizes in two crystal forms: one belongs to space group C222(1), with unit-cell parameters a = 102.4, b = 98.3, c = 47.9 A, and the other belongs to space group P2(1), with unit-cell parameters a = 77.3, b = 47.9, c = 71.9 A, beta = 89°. All these crystals diffract X-rays to better than 2.8 A resolution

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
GTPase domain of human Rheb recombinantly expressed in Escherichia coli	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
Rheb	-	Homo sapiens

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Release 2023.1 (January 2023)