Literature summary for 3.6.5.2 extracted from

Rittinger, K.; Walker, P.A.; Eccleston, J.F.; Smerdon, S.J.; Gamblin, S.J.
Structure at 1.65 A of RhoA and its GTPase-activating protein in complex with a transition-state analogue (1997), Nature, 389, 758-762.

Search for more literature for 3.6.5.2

Activating Compound

Activating Compound	Comment	Organism	Structure
GTPase activating protein GAP	RhoGAP, up to 1000fold activation of GTPase	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
1.65 A resolution, complex of RhoA-rhoGAP with the transition-state analogue GDPAIF4-, hanging drop vapour diffusion technique	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Homo sapiens	cycling between the active GTP bound and the inactive GDP bound state, slow GTPase activity	GDP + phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzymes, affinity chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	cycling between the active GTP bound and the inactive GDP bound state, slow GTPase activity	Homo sapiens	GDP + phosphate	-	ir

Synonyms

Synonyms	Comment	Organism
Rap1	-	Homo sapiens
RhoA	Rho subfamily	Homo sapiens

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Release 2023.1 (January 2023)