Protein Variants | Comment | Organism |
---|---|---|
D172A | the ration of (kcat/Km)ATP/(kcat/Km)GTP is 41% of the ratio determined for the wild-type enzyme | West Nile virus |
E169A | the ration of (kcat/Km)ATP/(kcat/Km)GTP is 38% of the ratio determined for the wild-type enzyme | West Nile virus |
E173A | the ration of (kcat/Km)ATP/(kcat/Km)GTP is 17% of the ratio determined for the wild-type enzyme | West Nile virus |
E180A | the ration of (kcat/Km)ATP/(kcat/Km)GTP is 35% of the ratio determined for the wild-type enzyme | West Nile virus |
E182A | the ration of (kcat/Km)ATP/(kcat/Km)GTP is 29% of the ratio determined for the wild-type enzyme | West Nile virus |
F179A | the ration of (kcat/Km)ATP/(kcat/Km)GTP is 19% of the ratio determined for the wild-type enzyme | West Nile virus |
K186A | the ration of (kcat/Km)ATP/(kcat/Km)GTP is 15% of the ratio determined for the wild-type enzyme | West Nile virus |
K187A | the ration of (kcat/Km)ATP/(kcat/Km)GTP is 8% of the ratio determined for the wild-type enzyme | West Nile virus |
Q188A | the ration of (kcat/Km)ATP/(kcat/Km)GTP is 33% of the ratio determined for the wild-type enzyme | West Nile virus |
R170A | the ration of (kcat/Km)ATP/(kcat/Km)GTP is 31% of the ratio determined for the wild-type enzyme | West Nile virus |
R185A | inactive mutant enzyme | West Nile virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',3'-dideoxy-ATP | - |
West Nile virus | |
2',3'-dideoxy-GTP | - |
West Nile virus | |
2'-deoxy-ATP | - |
West Nile virus | |
2'-deoxy-GTP | - |
West Nile virus | |
2'-deoxy-L-GTP | - |
West Nile virus | |
2'-fluoro-2'-deoxy-ATP | - |
West Nile virus | |
2-amino-ATP | - |
West Nile virus | |
2-hydroxy-ATP | - |
West Nile virus | |
3'-deoxy-ATP | - |
West Nile virus | |
6-methyl-thio-ITP | - |
West Nile virus | |
6-thio-GTP | - |
West Nile virus | |
7-methyl-GTP | - |
West Nile virus | |
8-bromo-ATP | - |
West Nile virus | |
ara-ATP | - |
West Nile virus | |
ATP | - |
West Nile virus | |
GTP | - |
West Nile virus | |
ITP | - |
West Nile virus | |
N1-methyl-ATP | - |
West Nile virus | |
N1-methyl-GTP | - |
West Nile virus | |
N6-methyl-ATP | - |
West Nile virus | |
ribavirin triphosphate | - |
West Nile virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
GTP | pH 7.5, 37°C, mutant enzyme F179A | West Nile virus | |
0.09 | - |
GTP | pH 7.5, 37°C, mutant enzyme K187A | West Nile virus | |
0.1 | - |
GTP | pH 7.5, 37°C, mutant enzyme K186A | West Nile virus | |
0.11 | - |
GTP | pH 7.5, 37°C, mutant enzyme E173A | West Nile virus | |
0.14 | - |
ATP | pH 7.5, 37°C, wild-type enzyme | West Nile virus | |
0.18 | - |
ATP | pH 7.5, 37°C, mutant enzyme D172A | West Nile virus | |
0.18 | - |
ATP | pH 7.5, 37°C, mutant enzyme F179A | West Nile virus | |
0.2 | - |
ATP | pH 7.5, 37°C, mutant enzyme E173A | West Nile virus | |
0.2 | - |
ATP | pH 7.5, 37°C, mutant enzyme R170A | West Nile virus | |
0.22 | - |
ATP | pH 7.5, 37°C, mutant enzyme E169A | West Nile virus | |
0.23 | - |
GTP | pH 7.5, 37°C, mutant enzyme E182A | West Nile virus | |
0.24 | - |
ATP | pH 7.5, 37°C, mutant enzyme E180A | West Nile virus | |
0.27 | - |
GTP | pH 7.5, 37°C, mutant enzyme E180A | West Nile virus | |
0.27 | - |
ATP | pH 7.5, 37°C, mutant enzyme E182A | West Nile virus | |
0.27 | - |
GTP | pH 7.5, 37°C, mutant enzyme R170A | West Nile virus | |
0.29 | - |
GTP | pH 7.5, 37°C, mutant enzyme Q188A | West Nile virus | |
0.33 | - |
GTP | pH 7.5, 37°C, mutant enzyme D172A | West Nile virus | |
0.34 | - |
GTP | pH 7.5, 37°C, mutant enzyme E169A | West Nile virus | |
0.35 | - |
GTP | pH 7.5, 37°C, wild-type enzyme | West Nile virus | |
0.39 | - |
ATP | pH 7.5, 37°C, mutant enzyme K186A | West Nile virus | |
0.64 | - |
ATP | pH 7.5, 37°C, mutant enzyme Q188A | West Nile virus | |
0.94 | - |
ATP | pH 7.5, 37°C, mutant enzyme K187A | West Nile virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | West Nile virus | the West Nile virus RNA helicase uses the energy derived from the hydrolysis of nucleotides to separate complementary strands of RNA | ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
West Nile virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
West Nile virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-dideoxy-ATP + H2O | 53% of the phosphohydrolase activity with ATP | West Nile virus | 2',3'-dideoxy-ADP + phosphate | - |
? | |
2',3'-dideoxy-GTP + H2O | 28% of the phosphohydrolase activity with ATP | West Nile virus | 2',3'-dideoxy-GDP + phosphate | - |
? | |
2'-deoxy-ATP + H2O | 62% of the phosphohydrolase activity with ATP | West Nile virus | 2'-deoxy-ADP + phosphate | - |
? | |
2'-deoxy-GTP + H2O | 39% of the phosphohydrolase activity with ATP | West Nile virus | 2'-deoxy-GDP + phosphate | - |
? | |
2'-deoxy-L-GTP + H2O | 11% of the phosphohydrolase activity with ATP | West Nile virus | 2'-deoxy-L-GDP + phosphate | - |
? | |
2'-fluoro-2'-deoxy-ATP + H2O | 63% of the phosphohydrolase activity with ATP | West Nile virus | 2'-fluoro-2'-deoxy-ADP + phosphate | - |
? | |
2'-fluoro-2'-deoxy-GTP + H2O | 22% of the phosphohydrolase activity with ATP | West Nile virus | 2'-fluoro-2'-deoxy-GDP + phosphate | - |
? | |
2'-O-methyl-GTP + H2O | 24% of the phosphohydrolase activity with ATP | West Nile virus | 2'-O-methyl-GDP + phosphate | - |
? | |
2-amino-ATP + H2O | 103% of the phosphohydrolase activity with ATP | West Nile virus | 2-amino-ADP + phosphate | - |
? | |
2-hydroxy-ATP + H2O | 40% of the phosphohydrolase activity with ATP | West Nile virus | 2-hydroxy-ADP + phosphate | - |
? | |
3'-deoxy-ATP + H2O | 60% of the phosphohydrolase activity with ATP | West Nile virus | 3'-deoxy-ADP + phosphate | - |
? | |
3'-deoxy-GTP + H2O | 12% of the phosphohydrolase activity with ATP | West Nile virus | 3'-deoxy-GDP + phosphate | - |
? | |
3'-O-methyl-GTP + H2O | 35% of the phosphohydrolase activity with ATP | West Nile virus | 3'-O-methyl-GDP + phosphate | - |
? | |
6-methyl-thio-GTP + H2O | 40% of the phosphohydrolase activity with ATP | West Nile virus | 6-methyl-thio-GDP + phosphate | - |
? | |
6-methyl-thio-ITP + H2O | 16% of the phosphohydrolase activity with ATP | West Nile virus | 6-methyl-thio-IDP + phosphate | - |
? | |
6-thio-GTP + H2O | 93% of the phosphohydrolase activity with ATP | West Nile virus | 6-thio-GDP + phosphate | - |
? | |
7-methyl-GTP + H2O | 14% of the phosphohydrolase activity with ATP | West Nile virus | 7-methyl-GDP + phosphate | - |
? | |
8-bromo-ATP + H2O | 124% of the phosphohydrolase activity with ATP | West Nile virus | 8-bromo-ADP + phosphate | - |
? | |
8-bromo-GTP + H2O | 19% of the phosphohydrolase activity with ATP | West Nile virus | 8-bromo-GDP + phosphate | - |
? | |
8-iodo-GTP + H2O | 54% of the phosphohydrolase activity with ATP | West Nile virus | 8-iodo-GDP + phosphate | - |
? | |
ara-ATP + H2O | 18% of the phosphohydrolase activity with ATP | West Nile virus | ara-ADP + phosphate | - |
? | |
ATP + H2O | the West Nile virus RNA helicase uses the energy derived from the hydrolysis of nucleotides to separate complementary strands of RNA | West Nile virus | ADP + phosphate | - |
? | |
ATP + H2O | the amino acids Arg185, Arg202 and Asn417 are critical for phosphohydrolysis | West Nile virus | ADP + phosphate | - |
? | |
GTP + H2O | 49% of the phosphohydrolase activity with ATP | West Nile virus | GDP + phosphate | - |
? | |
ITP + H2O | 49% of the phosphohydrolase activity with ATP | West Nile virus | IDP + phosphate | - |
? | |
N1-methyl-ATP + H2O | 66% of the phosphohydrolase activity with ATP | West Nile virus | N1-methyl-ADP + phosphate | - |
? | |
N1-methyl-GTP + H2O | 49% of the phosphohydrolase activity with ATP | West Nile virus | N1-methyl-GDP + phosphate | - |
? | |
N6-methyl-ATP + H2O | 43% of the phosphohydrolase activity with ATP | West Nile virus | N6-methyl-ADP + phosphate | - |
? | |
O6-methyl-GTP + H2O | 17% of the phosphohydrolase activity with ATP | West Nile virus | O6-methyl-GDP + phosphate | - |
? | |
ribavirin triphosphate + H2O | 36% of the phosphohydrolase activity with ATP | West Nile virus | ribavirin diphosphate + phosphate | - |
? | |
XTP + H2O | 40% of the phosphohydrolase activity with ATP | West Nile virus | XDP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NS3 protein | - |
West Nile virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | West Nile virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
ATP | pH 7.5, 37°C, mutant enzyme D172A | West Nile virus | |
0.92 | - |
GTP | pH 7.5, 37°C, mutant enzyme D172A | West Nile virus | |
0.92 | - |
ATP | pH 7.5, 37°C, mutant enzyme E173A | West Nile virus | |
1 | - |
GTP | pH 7.5, 37°C, mutant enzyme K187A | West Nile virus | |
1 | - |
ATP | pH 7.5, 37°C, mutant enzyme R170A | West Nile virus | |
1.1 | - |
GTP | pH 7.5, 37°C, mutant enzyme E173A | West Nile virus | |
1.1 | - |
ATP | pH 7.5, 37°C, mutant enzyme E180A | West Nile virus | |
1.1 | - |
GTP | pH 7.5, 37°C, mutant enzyme F179A | West Nile virus | |
1.2 | - |
ATP | pH 7.5, 37°C, mutant enzyme F179A | West Nile virus | |
1.25 | - |
ATP | pH 7.5, 37°C, mutant enzyme E169A | West Nile virus | |
1.3 | - |
GTP | pH 7.5, 37°C, mutant enzyme E180A | West Nile virus | |
1.7 | - |
GTP | pH 7.5, 37°C, mutant enzyme K186A | West Nile virus | |
1.7 | - |
GTP | pH 7.5, 37°C, mutant enzyme Q188A | West Nile virus | |
1.7 | - |
GTP | pH 7.5, 37°C, mutant enzyme R170A | West Nile virus | |
1.83 | - |
GTP | pH 7.5, 37°C, mutant enzyme E169A | West Nile virus | |
2.2 | - |
ATP | pH 7.5, 37°C, mutant enzyme K187A | West Nile virus | |
2.7 | - |
ATP | pH 7.5, 37°C, mutant enzyme K186A | West Nile virus | |
2.75 | - |
GTP | pH 7.5, 37°C, wild-type enzyme | West Nile virus | |
3 | - |
ATP | pH 7.5, 37°C, wild-type enzyme | West Nile virus | |
3.3 | - |
ATP | pH 7.5, 37°C, mutant enzyme E182A | West Nile virus | |
3.3 | - |
ATP | pH 7.5, 37°C, mutant enzyme Q188A | West Nile virus | |
3.6 | - |
GTP | pH 7.5, 37°C, mutant enzyme E182A | West Nile virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | West Nile virus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
2-amino-ATP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
0.3 | - |
ATP | pH 7.5, 37°C, wild-type enzyme | West Nile virus | |
0.3 | - |
2',3'-dideoxy-GTP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
0.4 | - |
2'-fluoro-2'-deoxy-ATP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
0.5 | - |
ara-ATP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
0.5 | - |
3'-deoxy-ATP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
0.5 | - |
N6-methyl-ATP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
0.6 | - |
2'-deoxy-ATP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
0.6 | - |
2',3'-dideoxy-ATP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
0.6 | - |
6-methyl-thio-ITP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
0.7 | - |
8-bromo-ATP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
0.9 | - |
ITP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
1 | - |
7-methyl-GTP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
1.3 | - |
2'-deoxy-L-GTP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
1.5 | - |
N1-methyl-ATP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
2.3 | - |
ribavirin triphosphate | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
2.4 | - |
2-hydroxy-ATP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
2.6 | - |
N1-methyl-GTP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
3.4 | - |
2'-deoxy-GTP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | |
8.1 | - |
GTP | pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.3 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | 2-amino-ATP | |
0.4 | - |
pH 7.5, 37°C, wild-type enzyme | West Nile virus | ATP | |
0.5 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | 2',3'-dideoxy-GTP | |
0.5 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | 2'-fluoro-2'-deoxy-ATP | |
0.7 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | ara-ATP | |
0.7 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | 3'-deoxy-ATP | |
0.7 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | N6-methyl-ATP | |
0.8 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | 2',3'-dideoxy-ATP | |
0.8 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | 6-methyl-thio-ITP | |
0.9 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | 2'-deoxy-ATP | |
0.9 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | 8-bromo-ATP | |
1.2 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | ITP | |
1.4 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | 7-methyl-GTP | |
1.8 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | 2'-deoxy-L-GTP | |
2 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | N1-methyl-ATP | |
2.7 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | 6-thio-GTP | |
3.1 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | ribavirin triphosphate | |
3.3 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | 2-hydroxy-ATP | |
3.5 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | N1-methyl-GTP | |
11 | - |
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction | West Nile virus | GTP |
General Information | Comment | Organism |
---|---|---|
physiological function | the West Nile virus RNA helicase uses the energy derived from the hydrolysis of nucleotides to separate complementary strands of RNA | West Nile virus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
ATP | pH 7.5, 37°C, mutant enzyme E180A | West Nile virus | |
2.3 | - |
ATP | pH 7.5, 37°C, mutant enzyme K187A | West Nile virus | |
2.79 | - |
GTP | pH 7.5, 37°C, mutant enzyme D172A | West Nile virus | |
3.22 | - |
ATP | pH 7.5, 37°C, mutant enzyme D172A | West Nile virus | |
4.6 | - |
ATP | pH 7.5, 37°C, mutant enzyme E173A | West Nile virus | |
4.8 | - |
GTP | pH 7.5, 37°C, mutant enzyme E180A | West Nile virus | |
5 | - |
ATP | pH 7.5, 37°C, mutant enzyme R170A | West Nile virus | |
5.2 | - |
ATP | pH 7.5, 37°C, mutant enzyme Q188A | West Nile virus | |
5.38 | - |
GTP | pH 7.5, 37°C, mutant enzyme E169A | West Nile virus | |
5.68 | - |
ATP | pH 7.5, 37°C, mutant enzyme E169A | West Nile virus | |
5.9 | - |
GTP | pH 7.5, 37°C, mutant enzyme Q188A | West Nile virus | |
6.3 | - |
GTP | pH 7.5, 37°C, mutant enzyme R170A | West Nile virus | |
6.7 | - |
ATP | pH 7.5, 37°C, mutant enzyme F179A | West Nile virus | |
6.9 | - |
ATP | pH 7.5, 37°C, mutant enzyme K186A | West Nile virus | |
10 | - |
GTP | pH 7.5, 37°C, mutant enzyme E173A | West Nile virus | |
11 | - |
GTP | pH 7.5, 37°C, mutant enzyme K187A | West Nile virus | |
12 | - |
GTP | pH 7.5, 37°C, mutant enzyme F179A | West Nile virus | |
12.2 | - |
ATP | pH 7.5, 37°C, mutant enzyme E182A | West Nile virus | |
15.7 | - |
GTP | pH 7.5, 37°C, mutant enzyme E182A | West Nile virus | |
17 | - |
GTP | pH 7.5, 37°C, mutant enzyme K186A | West Nile virus | |
21.43 | - |
ATP | pH 7.5, 37°C, wild-type enzyme | West Nile virus | |
22.26 | - |
GTP | pH 7.5, 37°C, wild-type enzyme | West Nile virus |