Protein Variants | Comment | Organism |
---|---|---|
A115C/D262C | site-directed mutagenesis, the mutant shows activity, structure and substrate specificity similar to the wild-type | Bacillus subtilis |
A115C/E224C | site-directed mutagenesis, the mutant shows activity, structure and substrate specificity similar to the wild-type | Bacillus subtilis |
A115C/S229C | site-directed mutagenesis, the mutant shows activity, structure and substrate specificity similar to the wild-type | Bacillus subtilis |
S108C/E224C | site-directed mutagenesis, the mutant shows activity, structure and substrate specificity similar to the wild-type | Bacillus subtilis |
S108C/S229C | site-directed mutagenesis, the mutant shows activity, structure and substrate specificity similar to the wild-type | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000114 | - |
RNA | pH 7.5, 37°C, recombinant YxiN mutant S108C/S229C | Bacillus subtilis | |
0.000156 | - |
RNA | pH 7.5, 37°C, native wild-type enzyme | Bacillus subtilis | |
0.00017 | - |
RNA | pH 7.5, 37°C, recombinant wild-type YxiN | Bacillus subtilis | |
0.000324 | - |
RNA | pH 7.5, 37°C, recombinant YxiN mutantA115C/S229C | Bacillus subtilis | |
0.000422 | - |
RNA | pH 7.5, 37°C, recombinant YxiN mutantA115C/D262C | Bacillus subtilis | |
0.000458 | - |
RNA | pH 7.5, 37°C, recombinant YxiN mutant S108C/E224C | Bacillus subtilis | |
0.000496 | - |
RNA | pH 7.5, 37°C, recombinant YxiN mutantA115C/E224C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Bacillus subtilis | - |
ADP + phosphate | - |
? | |
RNA + H2O | Bacillus subtilis | RNA unwinding activity, the enzyme contains two RecA-like domains, opening and closing of the interdomain cleft during RNA unwinding | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Bacillus subtilis | ADP + phosphate | - |
? | |
ATP + H2O | cooperative binding of ATP and RNA leads to a compact helicase structure | Bacillus subtilis | ADP + phosphate | - |
? | |
additional information | open helicase conformation in the absence of nucleotides, or in the presence of ATP, or ADP, or RNA. In the presence of ADP and RNA, the open conformation is retained. By contrast, cooperative binding of ATP and RNA leads to a compact helicase structure, direct transitions between open and closed conformations, overview | Bacillus subtilis | ? | - |
? | |
RNA + H2O | RNA unwinding activity, the enzyme contains two RecA-like domains, opening and closing of the interdomain cleft during RNA unwinding | Bacillus subtilis | ? | - |
? | |
RNA + H2O | RNA unwinding activity, substrate is a 154mer of 23S rRNA generated by T7 polymerase from in vitro transcription | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DEAD box helicase | - |
Bacillus subtilis |
DEAD box RNA helicase | - |
Bacillus subtilis |
RNA helicase | - |
Bacillus subtilis |
YxiN | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.64 | - |
RNA | pH 7.5, 37°C, recombinant YxiN mutant S108C/S229C | Bacillus subtilis | |
0.85 | - |
RNA | pH 7.5, 37°C, recombinant YxiN mutant S108C/E224C | Bacillus subtilis | |
0.98 | - |
RNA | pH 7.5, 37°C, recombinant wild-type YxiN | Bacillus subtilis | |
1.3 | - |
RNA | pH 7.5, 37°C, recombinant YxiN mutantA115C/S229C | Bacillus subtilis | |
1.4 | - |
RNA | pH 7.5, 37°C, recombinant YxiN mutantA115C/E224C | Bacillus subtilis | |
1.41 | - |
RNA | pH 7.5, 37°C, recombinant YxiN mutantA115C/D262C | Bacillus subtilis | |
1.48 | - |
RNA | pH 7.5, 37°C, native wild-type enzyme | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus subtilis |