Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.4.13 extracted from

  • Theissen, B.; Karow, A.R.; Koehler, J.; Gubaev, A.; Klostermeier, D.
    Cooperative binding of ATP and RNA induces a closed conformation in a DEAD box RNA helicase (2008), Proc. Natl. Acad. Sci. USA, 105, 548-553.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
A115C/D262C site-directed mutagenesis, the mutant shows activity, structure and substrate specificity similar to the wild-type Bacillus subtilis
A115C/E224C site-directed mutagenesis, the mutant shows activity, structure and substrate specificity similar to the wild-type Bacillus subtilis
A115C/S229C site-directed mutagenesis, the mutant shows activity, structure and substrate specificity similar to the wild-type Bacillus subtilis
S108C/E224C site-directed mutagenesis, the mutant shows activity, structure and substrate specificity similar to the wild-type Bacillus subtilis
S108C/S229C site-directed mutagenesis, the mutant shows activity, structure and substrate specificity similar to the wild-type Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000114
-
RNA pH 7.5, 37°C, recombinant YxiN mutant S108C/S229C Bacillus subtilis
0.000156
-
RNA pH 7.5, 37°C, native wild-type enzyme Bacillus subtilis
0.00017
-
RNA pH 7.5, 37°C, recombinant wild-type YxiN Bacillus subtilis
0.000324
-
RNA pH 7.5, 37°C, recombinant YxiN mutantA115C/S229C Bacillus subtilis
0.000422
-
RNA pH 7.5, 37°C, recombinant YxiN mutantA115C/D262C Bacillus subtilis
0.000458
-
RNA pH 7.5, 37°C, recombinant YxiN mutant S108C/E224C Bacillus subtilis
0.000496
-
RNA pH 7.5, 37°C, recombinant YxiN mutantA115C/E224C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Bacillus subtilis
-
ADP + phosphate
-
?
RNA + H2O Bacillus subtilis RNA unwinding activity, the enzyme contains two RecA-like domains, opening and closing of the interdomain cleft during RNA unwinding ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Bacillus subtilis ADP + phosphate
-
?
ATP + H2O cooperative binding of ATP and RNA leads to a compact helicase structure Bacillus subtilis ADP + phosphate
-
?
additional information open helicase conformation in the absence of nucleotides, or in the presence of ATP, or ADP, or RNA. In the presence of ADP and RNA, the open conformation is retained. By contrast, cooperative binding of ATP and RNA leads to a compact helicase structure, direct transitions between open and closed conformations, overview Bacillus subtilis ?
-
?
RNA + H2O RNA unwinding activity, the enzyme contains two RecA-like domains, opening and closing of the interdomain cleft during RNA unwinding Bacillus subtilis ?
-
?
RNA + H2O RNA unwinding activity, substrate is a 154mer of 23S rRNA generated by T7 polymerase from in vitro transcription Bacillus subtilis ?
-
?

Synonyms

Synonyms Comment Organism
DEAD box helicase
-
Bacillus subtilis
DEAD box RNA helicase
-
Bacillus subtilis
RNA helicase
-
Bacillus subtilis
YxiN
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.64
-
RNA pH 7.5, 37°C, recombinant YxiN mutant S108C/S229C Bacillus subtilis
0.85
-
RNA pH 7.5, 37°C, recombinant YxiN mutant S108C/E224C Bacillus subtilis
0.98
-
RNA pH 7.5, 37°C, recombinant wild-type YxiN Bacillus subtilis
1.3
-
RNA pH 7.5, 37°C, recombinant YxiN mutantA115C/S229C Bacillus subtilis
1.4
-
RNA pH 7.5, 37°C, recombinant YxiN mutantA115C/E224C Bacillus subtilis
1.41
-
RNA pH 7.5, 37°C, recombinant YxiN mutantA115C/D262C Bacillus subtilis
1.48
-
RNA pH 7.5, 37°C, native wild-type enzyme Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Bacillus subtilis