Literature summary for 3.6.4.13 extracted from

Hartjen, P.; Medom, B.K.; Reinholz, M.; Borowski, P.; Baier, A.
Regulation of the biochemical function of motif VI of HCV NTPase/helicase by the conserved Phe-loop (2009), Biochimie, 91, 252-260.

Search for more literature for 3.6.4.13

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3), recombinant protein, NS3d2wt variant corresponding to wild-type domain 2, NS3d2D construct comprises the complete domain, HCV(1361-1503) without loop, pET21b andpET16b vectors	Hepacivirus C
expression of the Arg-rich amino acid motif HCV1487-1500, of the complete domain 2, and of domain 2 lacking the flexible loop localized between Val1458 and Thr1476 as His10-tagged proteins in Escherichia coli strain BL21(DE3)	Hepacivirus C

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	domain 2 of wild-type NS3 protein and domain 2 devoid of the loop structure used for inhibition studies on functions of protein kinase C (PKC), inhibitory potential towards the majority of protein kinase C isoforms shown	Hepacivirus C

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O	Hepacivirus C	the Arg-rich amino acid motif HCV1487-1500, a fragment of domain 2 NS3 of Hepatitis C virus, as well as the complete domain 2, and domain 2 lacking the flexible loop localized between Val1458 and Thr1476, mediate competitive inhibition of diverse protein kinase C functions, inhibition of rat brain PKC, overview	ADP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Hepacivirus C	P27958	-	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration, SDS-PAGE	Hepacivirus C
recombinant His10-tagged Arg-rich amino acid motif HCV1487-1500, complete domain 2, and domain 2 lacking the flexible loop from Escherichia coli strain BL21(DE3)	Hepacivirus C

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	surface of domain 2 of the NS3 NTPase/helicase in direct vicinity to a flexible loop that is localized between Val1458 and Thr1476, accessibility of the Arg-rich amino acid motif by this loop for protein kinase C inhibition analyzed, two variants of domain 2 generated, in vitro protein kinase C (PKC) phosphorylation studies, binding and competition assays, modelling of ribbon diagrams, presence of the intact loop abolishes the binding of domain 2 to a tailed duplex RNA, binding of dsDNA not affected, loop structure reduces the extent of inhibition of protein kinase C (PKC) by domain 2 and regulates the binding of dsRNA, various mechanisms by which the NS3 protein perturb signal transduction in infected cells	Hepacivirus C

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Hepacivirus C	ADP + phosphate	-	?
ATP + H2O	the Arg-rich amino acid motif HCV1487-1500, a fragment of domain 2 NS3 of Hepatitis C virus, as well as the complete domain 2, and domain 2 lacking the flexible loop localized between Val1458 and Thr1476, mediate competitive inhibition of diverse protein kinase C functions, inhibition of rat brain PKC, overview	Hepacivirus C	ADP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
nonstructural protein 3	-	Hepacivirus C
nonstructural protein 3	ambiguous	Hepacivirus C
NS3	-	Hepacivirus C
NS3 protein	ambiguous	Hepacivirus C
NTPase/helicase	-	Hepacivirus C

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Release 2023.1 (January 2023)