Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.4.13 extracted from

  • Lee, C.G.; Hurwitz, J.
    A new RNA helicase isolated from HeLa cells that catalytically translocates in the 3' to 5' direction. (1992), J. Biol. Chem., 267, 4398-4407.
    View publication on PubMed
Search for more literature for 3.6.4.13

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Homo sapiens
KCl slight stimulation at 0.05-0.1 M, inhibition at 0.2 M Homo sapiens
NEM
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0157
-
 ATP pH 7.6, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
KCl slight stimulation at 0.05-0.1 M, inhibition at 0.2 M Homo sapiens
Mg2+ divalent cation required, Ca2+ or Mn2+ do not substitute for Mg2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 glycerol gradient centrifugation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O RNA helicase A utilizes all hydrolyzable NTPs without preference. RNA helicase A unwinds dsRNA only in a 3' to 5' direction. The enzyme can only translocate on RNA possessing 3’ single-stranded regions Homo sapiens ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 130000, SDS-PAGE Homo sapiens