Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for development of specific antiviral inhibitors | Dengue virus |
Crystallization (Comment) | Organism |
---|---|
purified catalytic domain fragment, hanging drop vapour diffusion method, 0.002 ml of 10 mg/ml protein in 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate, 14% PEG 8000, mixed with an equal volume of precipitation solution, 18°C, macroseeding, cryoprotection by 25% glycerol, X-ray diffraction structure determination and analysis at 2.4 A resolution, modeling | Dengue virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | metal-dependent NTPase activity, mechanism involves a bound sulfate ion | Dengue virus | |
sulfate | bound, required for the metal-dependent NTPase reaction mechanism | Dengue virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Dengue virus | RNA helicase activity | ADP + phosphate | - |
? | |
additional information | Dengue virus | the enzyme plays an important role in viral replication | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dengue virus | - |
i.e. DNV | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a nucleoside triphosphate + H2O = a nucleoside diphosphate + phosphate | catalytic mechanism involving a bound sulfate ion, NTPase active site structure, nucleic acid binding site | Dengue virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | RNA helicase activity | Dengue virus | ADP + phosphate | - |
? | |
additional information | the enzyme plays an important role in viral replication | Dengue virus | ? | - |
? | |
additional information | multifunctional enzyme showing protease, helicase, and NTPase activities | Dengue virus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystal structure, three-domain structure with asymmetric distribution of charges on the surface and a tunnel structure for RNA substrate access, overview | Dengue virus |
Synonyms | Comment | Organism |
---|---|---|
helicase/nucleoside triphosphatase | - |
Dengue virus |
NS3 | - |
Dengue virus |
nucleoside 5'-triphosphatase | - |
Dengue virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Dengue virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Dengue virus |