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Literature summary for 3.6.4.13 extracted from
- Characterization of ATPase activity of a hepatitis C virus NS3 helicase domain, and analysis involving mercuric reagents (2003), J. Biochem., 134, 505-511.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzyme is a target for anti-HCV drug development | Hepacivirus C |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of C-terminally His-tagged truncated NS3 NTPase/helicase domain in Escherichia coli | Hepacivirus C |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | inhibits ATPase activity, IC50: 0.13 mM | Hepacivirus C |  |
| Fe2+ | inhibits ATPase activity, IC50: 0.75 mM | Hepacivirus C | |
| Hg2+ | inhibits ATPase activity, IC50: 49 nM, targets the cysteine residue in the DECH box, competitive, cysteine or DTT protect at large concentrations | Hepacivirus C | |
| additional information | no or poor inhibition by Mn2+, Zn2+, Co2+, Ca2+, and Ni2+ | Hepacivirus C | |
| PCMB | inhibits ATPase activity, IC50: 88 nM | Hepacivirus C | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | preferred metal ion, ATPase activity | Hepacivirus C | |
| Mn2+ | can substitute partially for Mg2+, ATPase activity | Hepacivirus C | |
| additional information | no or poor effect by Mn2+, Zn2+, Co2+, Ca2+, and Ni2+ | Hepacivirus C |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Hepacivirus C | the enzyme is involved in viral replication | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hepacivirus C | - |
i.e. HCV | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His-tagged truncated NS3 NTPase/helicase domain from Escherichia coli by nickel affinity chromatography | Hepacivirus C |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | preferred substrate for NTPase activity | Hepacivirus C | ADP + phosphate | - |
? | |
| CTP + H2O | NTPase activity | Hepacivirus C | CDP + phosphate | - |
? | |
| GTP + H2O | NTPase activity | Hepacivirus C | GDP + phosphate | - |
? | |
| additional information | the enzyme is involved in viral replication | Hepacivirus C | ? | - |
? | |
| additional information | the multifunctional enzyme shows RNA-dependent NTPase and helicase activities, no activity with ADP and AMP | Hepacivirus C | ? | - |
? | |
| UTP + H2O | NTPase activity | Hepacivirus C | UDP + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATPase | - |
Hepacivirus C |
| nonstructural protein 3 | - |
Hepacivirus C |
| NS3 | - |
Hepacivirus C |
| RNA-dependent NTPase/helicase | - |
Hepacivirus C |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
ATPase activity | Hepacivirus C |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | activity range, ATPase activity | Hepacivirus C |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.000049 | - |
inhibits ATPase activity, IC50: 49 nM, targets the cysteine residue in the DECH box, competitive, cysteine or DTT protect at large concentrations | Hepacivirus C | Hg2+ | |
| 0.000088 | - |
inhibits ATPase activity, IC50: 88 nM | Hepacivirus C | PCMB | |
| 0.13 | - |
inhibits ATPase activity, IC50: 0.13 mM | Hepacivirus C | Cu2+ | |
| 0.75 | - |
inhibits ATPase activity, IC50: 0.75 mM | Hepacivirus C | Fe2+ | |
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