Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cardiolipin | - |
Escherichia coli | |
diacylglycerol diphosphate | increases the activity with undecaprenyl diphosphate by about 400% | Escherichia coli | |
phosphatidylglycerol | - |
Escherichia coli | |
Phospholipids | - |
Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene pgpB, overexpression of His-tagged enzyme in strain DH5alpha and C43 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
diacylglycerol diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
0.096 | - |
farnesyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
0.53 | - |
undecaprenyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
1.7 | - |
phosphatidic acid | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
3.6 | - |
isopentenyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
3.9 | - |
diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral, PgpB contains six transmembrane segments, a large periplasmic loop, and the type 2 phosphatidic acid phosphatase signature residues at a periplasmic location | Escherichia coli | 16020 | - |
additional information | topological mapping, modelling, overview | Escherichia coli | - |
- |
periplasm | PgpB contains six transmembrane segments, a large periplasmic loop, and the type 2 phosphatidic acid phosphatase signature residues at a periplasmic location | Escherichia coli | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30158, mass spectrometry, x * 30000, SDS-PAGE | Escherichia coli |
30158 | - |
x * 30158, mass spectrometry, x * 30000, SDS-PAGE | Escherichia coli |
110000 | - |
recombinant His-tagged enzyme, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
undecaprenyl diphosphate + H2O | Escherichia coli | the synthesis of the lipid carrier undecaprenyl phosphate, C55-P, requires the dephosphorylation of its precursor, undecaprenyl pyrophosphate, C55-PP, the dephosphorylation involves four integral membrane proteins, BacA, and three members of the type 2 phosphatidic acid phosphatase family, PgpB, YbjG, and YeiU, overview | undecaprenyl phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from membranes to homogeneity by ultracentrifugation, nickel affinity chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
purified recombinant enzyme, substrate isopentenyl diphosphate | Escherichia coli |
3 | - |
purified recombinant enzyme, substrate undecaprenyl diphosphate | Escherichia coli |
6.7 | - |
purified recombinant enzyme, substrate phosphatidic acid | Escherichia coli |
300 | - |
purified recombinant enzyme, substrate farnesyl diphosphate | Escherichia coli |
310 | - |
purified recombinant enzyme, substrate diacylglycerol diphosphate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacylglycerol diphosphate + H2O | - |
Escherichia coli | diacylglycerol phosphate + phosphate | - |
? | |
diphosphate + H2O | low activity | Escherichia coli | 2 phosphate | - |
? | |
farnesyl diphosphate + H2O | - |
Escherichia coli | farnesyl phosphate + phosphate | - |
? | |
isopentenyl diphosphate + H2O | low activity | Escherichia coli | isopentenyl phosphate + phosphate | - |
? | |
additional information | substrate specificity, overview | Escherichia coli | ? | - |
? | |
phosphatidic acid + H2O | low activity | Escherichia coli | ? | - |
? | |
undecaprenyl diphosphate + H2O | low activity | Escherichia coli | undecaprenyl phosphate + phosphate | - |
? | |
undecaprenyl diphosphate + H2O | the synthesis of the lipid carrier undecaprenyl phosphate, C55-P, requires the dephosphorylation of its precursor, undecaprenyl pyrophosphate, C55-PP, the dephosphorylation involves four integral membrane proteins, BacA, and three members of the type 2 phosphatidic acid phosphatase family, PgpB, YbjG, and YeiU, overview | Escherichia coli | undecaprenyl phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme exists as a monomer in n-dodecyl-beta-D-maltoside solution, topological mapping, overview | Escherichia coli |
oligomer | x * 30158, mass spectrometry, x * 30000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a member of the type 2 phosphatidic acid phosphatase family | Escherichia coli |
PgpB | - |
Escherichia coli |
undecaprenyl pyrophosphate phosphatase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
undecaprenyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
19 | - |
isopentenyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
48 | - |
diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
61 | - |
phosphatidic acid | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
280 | - |
diacylglycerol diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
290 | - |
farnesyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | 50% of maximal activity at pH values other than pH 6.5-pH 7.5 | Escherichia coli |