Cloned (Comment) | Organism |
---|---|
- |
Mason-Pfizer monkey virus |
Crystallization (Comment) | Organism |
---|---|
in complex with nucleotides. Reaction starts with dUTPase capturing and properly positioning two substrates: dUTP and the catalytic water molecule. the catalytic water molecule is placed near the alpha-phosphate group opposite from the leaving group. Hydrolysis is then initiated by nucleophilic attack of Wthe water on the alpha-phosphate along the line of the scissile bond (in-line attack). A symmetric phosphorane-like transition state structure is formed with significant bond order in both forming and breaking bonds. Bond breaking is concomitant with inversion of the alpha-phosphate configuration and diphosphate-Mg2+ escape. The dUMP product then relaxes by rotation of the alpha-phosphate group and establishes a configuration similar to the one observed for this moiety in the E-S complex | Mason-Pfizer monkey virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mason-Pfizer monkey virus | O92810 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,beta-imido-dUTP + H2O | - |
Mason-Pfizer monkey virus | dUMP + amino-diphosphate | - |
? | |
dUTP + H2O | - |
Mason-Pfizer monkey virus | dUMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pr95 | - |
Mason-Pfizer monkey virus |