Application | Comment | Organism |
---|---|---|
medicine | dUTPase is a target for antitubercular drugs | Mycobacterium tuberculosis |
medicine | dUTPase is a target for antitubercular drugs | Mycolicibacterium smegmatis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) (pLysS) cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the DELTA-loop deletion mutant is created by deletion of the five (Ala133-Ser137) loop-specific amino acids and shows reduced kcat and increased Km values for dUTP | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
dUTP | wild type enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C | Mycobacterium tuberculosis | |
0.0011 | - |
dUTP | DELTA-loop mutant enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Mycobacterium tuberculosis | - |
dUMP + diphosphate | - |
? | |
dUTP + H2O | Mycolicibacterium smegmatis | - |
dUMP + diphosphate | - |
? | |
dUTP + H2O | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
dUMP + diphosphate | - |
? | |
dUTP + H2O | Mycobacterium tuberculosis H37Rv | - |
dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNS5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WNS5 | - |
- |
Mycolicibacterium smegmatis | A0QW08 | - |
- |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | A0QW08 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Mycobacterium tuberculosis |
Ni-NTA column chromatography | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | - |
Mycobacterium tuberculosis | dUMP + diphosphate | - |
? | |
dUTP + H2O | - |
Mycolicibacterium smegmatis | dUMP + diphosphate | - |
? | |
dUTP + H2O | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | dUMP + diphosphate | - |
? | |
dUTP + H2O | - |
Mycobacterium tuberculosis H37Rv | dUMP + diphosphate | - |
? | |
additional information | the enzyme cannot hydrolyze alpha-beta-imido-dUTP | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the enzyme cannot hydrolyze alpha-beta-imido-dUTP | Mycolicibacterium smegmatis | ? | - |
? | |
additional information | the enzyme cannot hydrolyze alpha-beta-imido-dUTP | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? | |
additional information | the enzyme cannot hydrolyze alpha-beta-imido-dUTP | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deoxyuridine 5-triphosphate nucleotidohydrolase | - |
Mycobacterium tuberculosis |
deoxyuridine 5-triphosphate nucleotidohydrolase | - |
Mycolicibacterium smegmatis |
DUT | - |
Mycobacterium tuberculosis |
DUT | - |
Mycolicibacterium smegmatis |
dUTPase | - |
Mycobacterium tuberculosis |
dUTPase | - |
Mycolicibacterium smegmatis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.88 | - |
dUTP | DELTA-loop mutant enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C | Mycobacterium tuberculosis | |
1.22 | - |
dUTP | wild type enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
malfunction | dUTPase knock-out results in lethality | Mycolicibacterium smegmatis |