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Literature summary for 3.6.1.23 extracted from

  • Quesada-Soriano, I.; Casas-Solvas, J.M.; Recio, E.; Ruiz-Perez, L.M.; Vargas-Berenguel, A.; Gonzalez-Pacanowska, D.; Garcia-Fuentes, L.
    Kinetic properties and specificity of trimeric Plasmodium falciparum and human dUTPases (2010), Biochimie, 92, 178-186.
    View publication on PubMed
Search for more literature for 3.6.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
dTMP
-
 Homo sapiens
dTMP
-
 Plasmodium falciparum
fluorodeoxy-UMP
-
 Homo sapiens
fluorodeoxy-UMP
-
 Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
0.00173
-
 2'-deoxy-5-fluoro-UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
0.00187
-
 dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
0.0061
-
 2'-deoxy-5-fluoro-UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
0.29
-
 dTTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
0.438
-
 beta-L-2'-dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
0.465
-
 dCTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
0.523
-
 beta-L-2'-dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
0.54
-
 UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
0.571
-
 UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
0.687
-
 dCTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
0.761
-
 dTTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
1.13
-
 dATP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
1.96
-
 dGTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
-
 Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxy-5-fluoro-UTP + H2O most specific substrate Homo sapiens 2'-deoxy-5-fluoro-UMP + diphosphate
-
 ?
2'-deoxy-5-fluoro-UTP + H2O most specific substrate Plasmodium falciparum 2'-deoxy-5-fluoro-UMP + diphosphate
-
 ?
beta-L-2'-dUTP + H2O
-
 Homo sapiens beta-L-2'-dUMP + diphosphate
-
 ?
beta-L-2'-dUTP + H2O
-
 Plasmodium falciparum beta-L-2'-dUMP + diphosphate
-
 ?
dATP + H2O
-
 Homo sapiens dAMP + diphosphate
-
 ?
dATP + H2O
-
 Plasmodium falciparum dAMP + diphosphate
-
 ?
dCTP + H2O
-
 Homo sapiens dCMP + diphosphate
-
 ?
dCTP + H2O
-
 Plasmodium falciparum dCMP + diphosphate
-
 ?
dGTP + H2O
-
 Homo sapiens dGMP + diphosphate
-
 ?
dGTP + H2O
-
 Plasmodium falciparum dGMP + diphosphate
-
 ?
dTTP + H2O
-
 Homo sapiens dTMP + diphosphate
-
 ?
dTTP + H2O
-
 Plasmodium falciparum dTMP + diphosphate
-
 ?
dUTP + H2O most specific substrate Homo sapiens dUMP + diphosphate
-
 ?
dUTP + H2O most specific substrate Plasmodium falciparum dUMP + diphosphate
-
 ?
UTP + H2O
-
 Homo sapiens UMP + diphosphate
-
 ?
UTP + H2O
-
 Plasmodium falciparum UMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
deoxyuridine 5'-triphosphate nucleotidohydrolase
-
 Homo sapiens
deoxyuridine 5'-triphosphate nucleotidohydrolase
-
 Plasmodium falciparum
dUTPase
-
 Homo sapiens
dUTPase
-
 Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00013
-
 dGTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
0.00265
-
 dATP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
0.00525
-
 beta-L-2'-dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
0.0061
-
 beta-L-2'-dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
0.0092
-
 dTTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
0.01121
-
 UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
0.01278
-
 UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
0.01971
-
 dTTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
0.02172
-
 dCTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
0.1616
-
 dCTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
110
-
 2'-deoxy-5-fluoro-UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
600
-
 2'-deoxy-5-fluoro-UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
6600
-
 dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
7100
-
 dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum