Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for drugs against campylobacteriosis | Campylobacter jejuni |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli strain BL21(DE3) | Campylobacter jejuni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-deoxy-5'-phenylmethylaminouracil | - |
Homo sapiens | |
2'-deoxy-5'-triphenylmethanyluracil | - |
Homo sapiens | |
2'-deoxyuracil 5'-diphenyl phosphate | - |
Homo sapiens | |
3-[5-(trityloxy)tetrahydrofuran-2-yl]pyridine-2,6(1H,3H)-dione | - |
Homo sapiens | |
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2',3'-dideoxyuracil | - |
Homo sapiens | |
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2'-deoxyuracil | - |
Homo sapiens | |
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-3'-methyl-2',3'-dideoxyuracil | - |
Homo sapiens | |
5'-(2,3,3-trimethylbutan-2-yl)-3'-methyl-2',3'-dideoxyuracil | - |
Homo sapiens | |
5'-(2,4-dimethyl-3-(1-methylethyl)pentan-3-yl)-2'-deoxyuracil | - |
Homo sapiens | |
5'-adamantoyl-3'-methyl-2',3'-dideoxyuracil | - |
Homo sapiens | |
5'-hexadecanoyl-3'-methyl-2',3'-dideoxyuracil | - |
Homo sapiens | |
alpha,beta-imido-dUDP | potent inhibition | Campylobacter jejuni | |
dUMP | product inhibition | Campylobacter jejuni | |
dUMP | product inhibition | Homo sapiens | |
additional information | several dUMP analogues with substituents at the 3'- or 5'-positions show poor inhibitory activity | Campylobacter jejuni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, kinetics analysis | Campylobacter jejuni | |
0.00066 | - |
dUTP | pH 8.0, 25°C, recombinant enzyme | Campylobacter jejuni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Homo sapiens | |
Mg2+ | activates | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Homo sapiens | dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes | dUMP + diphosphate | - |
? | |
dUTP + H2O | Campylobacter jejuni | dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes | dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by hydroxyapatite chromatography, and two steps of anion exchange chromatography | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUDP + H2O | the enzyme is highly specific for deoxyuridine nucleotides | Homo sapiens | deoxyuridine + diphosphate | - |
? | |
dUDP + H2O | the enzyme is highly specific for deoxyuridine nucleotides | Campylobacter jejuni | deoxyuridine + diphosphate | - |
? | |
dUTP + H2O | dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes | Homo sapiens | dUMP + diphosphate | - |
? | |
dUTP + H2O | dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes | Campylobacter jejuni | dUMP + diphosphate | - |
? | |
dUTP + H2O | the enzyme is highly specific for deoxyuridine nucleotides | Homo sapiens | dUMP + diphosphate | - |
? | |
dUTP + H2O | the enzyme is highly specific for deoxyuridine nucleotides | Campylobacter jejuni | dUMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
deoxyuridine 5'-triphosphate nucleotidohydrolase | - |
Homo sapiens |
deoxyuridine 5'-triphosphate nucleotidohydrolase | - |
Campylobacter jejuni |
dUTPase | - |
Homo sapiens |
dUTPase | - |
Campylobacter jejuni |
dUTPase-dUDPase | - |
Homo sapiens |
dUTPase-dUDPase | - |
Campylobacter jejuni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
25 | - |
assay at | Campylobacter jejuni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.3 | - |
dUTP | pH 8.0, 25°C, recombinant enzyme | Campylobacter jejuni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
8 | - |
assay at | Campylobacter jejuni |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2',3'-dideoxyuracil | above 1 mM, pH 8.0, 25°C | Homo sapiens | |
additional information | - |
5'-(2,3,3-trimethylbutan-2-yl)-3'-methyl-2',3'-dideoxyuracil | above 1 mM, pH 8.0, 25°C | Homo sapiens | |
additional information | - |
5'-(2,4-dimethyl-3-(1-methylethyl)pentan-3-yl)-2'-deoxyuracil | above 1 mM, pH 8.0, 25°C | Homo sapiens | |
additional information | - |
2'-deoxy-5'-phenylmethylaminouracil | above 1 mM, pH 8.0, 25°C | Homo sapiens | |
0.0114 | - |
dUMP | pH 8.0, 25°C, recombinant enzyme | Campylobacter jejuni | |
0.135 | - |
2'-deoxyuracil 5'-diphenyl phosphate | pH 8.0, 25°C | Homo sapiens | |
0.156 | - |
3-[5-(trityloxy)tetrahydrofuran-2-yl]pyridine-2,6(1H,3H)-dione | pH 8.0, 25°C | Homo sapiens | |
0.298 | - |
5'-adamantoyl-3'-methyl-2',3'-dideoxyuracil | pH 8.0, 25°C | Homo sapiens | |
0.35 | - |
5'-hexadecanoyl-3'-methyl-2',3'-dideoxyuracil | pH 8.0, 25°C | Homo sapiens | |
0.547 | - |
5'-adamantoyl-3'-methyl-2',3'-dideoxyuracil | pH 8.0, 25°C, recombinant enzyme | Homo sapiens | |
0.805 | - |
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2'-deoxyuracil | pH 8.0, 25°C | Homo sapiens | |
0.807 | - |
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-3'-methyl-2',3'-dideoxyuracil | pH 8.0, 25°C | Homo sapiens | |
0.908 | - |
2'-deoxy-5'-triphenylmethanyluracil | pH 8.0, 25°C | Homo sapiens |