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Literature summary for 3.6.1.23 extracted from

  • Musso-Buendia, J.A.; Vidal, A.E.; Kasinthan, G.; Nguyen, C.; Carrero-Lerida, J.; Ruiz-Perez, L.M.; Wilson, K.; Johansson, N.G.; Gilbert, I.H.; Gonzalez-Pacanowska, D.
    Kinetic properties and inhibition of the dimeric dUTPase-dUDPase from Campylobacter jejuni (2009), J. Enzyme Inhib. Med. Chem., 24, 111-116.
    View publication on PubMed

Application

Application Comment Organism
drug development the enzyme is a target for drugs against campylobacteriosis Campylobacter jejuni

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli strain BL21(DE3) Campylobacter jejuni

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxy-5'-phenylmethylaminouracil
-
Homo sapiens
2'-deoxy-5'-triphenylmethanyluracil
-
Homo sapiens
2'-deoxyuracil 5'-diphenyl phosphate
-
Homo sapiens
3-[5-(trityloxy)tetrahydrofuran-2-yl]pyridine-2,6(1H,3H)-dione
-
Homo sapiens
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2',3'-dideoxyuracil
-
Homo sapiens
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2'-deoxyuracil
-
Homo sapiens
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-3'-methyl-2',3'-dideoxyuracil
-
Homo sapiens
5'-(2,3,3-trimethylbutan-2-yl)-3'-methyl-2',3'-dideoxyuracil
-
Homo sapiens
5'-(2,4-dimethyl-3-(1-methylethyl)pentan-3-yl)-2'-deoxyuracil
-
Homo sapiens
5'-adamantoyl-3'-methyl-2',3'-dideoxyuracil
-
Homo sapiens
5'-hexadecanoyl-3'-methyl-2',3'-dideoxyuracil
-
Homo sapiens
alpha,beta-imido-dUDP potent inhibition Campylobacter jejuni
dUMP product inhibition Campylobacter jejuni
dUMP product inhibition Homo sapiens
additional information several dUMP analogues with substituents at the 3'- or 5'-positions show poor inhibitory activity Campylobacter jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, kinetics analysis Campylobacter jejuni
0.00066
-
dUTP pH 8.0, 25°C, recombinant enzyme Campylobacter jejuni

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Homo sapiens
Mg2+ activates Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dUTP + H2O Homo sapiens dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes dUMP + diphosphate
-
?
dUTP + H2O Campylobacter jejuni dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes dUMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by hydroxyapatite chromatography, and two steps of anion exchange chromatography Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUDP + H2O the enzyme is highly specific for deoxyuridine nucleotides Homo sapiens deoxyuridine + diphosphate
-
?
dUDP + H2O the enzyme is highly specific for deoxyuridine nucleotides Campylobacter jejuni deoxyuridine + diphosphate
-
?
dUTP + H2O dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes Homo sapiens dUMP + diphosphate
-
?
dUTP + H2O dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes Campylobacter jejuni dUMP + diphosphate
-
?
dUTP + H2O the enzyme is highly specific for deoxyuridine nucleotides Homo sapiens dUMP + diphosphate
-
?
dUTP + H2O the enzyme is highly specific for deoxyuridine nucleotides Campylobacter jejuni dUMP + diphosphate
-
?

Subunits

Subunits Comment Organism
dimer
-
Campylobacter jejuni

Synonyms

Synonyms Comment Organism
deoxyuridine 5'-triphosphate nucleotidohydrolase
-
Homo sapiens
deoxyuridine 5'-triphosphate nucleotidohydrolase
-
Campylobacter jejuni
dUTPase
-
Homo sapiens
dUTPase
-
Campylobacter jejuni
dUTPase-dUDPase
-
Homo sapiens
dUTPase-dUDPase
-
Campylobacter jejuni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens
25
-
assay at Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.3
-
dUTP pH 8.0, 25°C, recombinant enzyme Campylobacter jejuni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens
8
-
assay at Campylobacter jejuni

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2',3'-dideoxyuracil above 1 mM, pH 8.0, 25°C Homo sapiens
additional information
-
5'-(2,3,3-trimethylbutan-2-yl)-3'-methyl-2',3'-dideoxyuracil above 1 mM, pH 8.0, 25°C Homo sapiens
additional information
-
5'-(2,4-dimethyl-3-(1-methylethyl)pentan-3-yl)-2'-deoxyuracil above 1 mM, pH 8.0, 25°C Homo sapiens
additional information
-
2'-deoxy-5'-phenylmethylaminouracil above 1 mM, pH 8.0, 25°C Homo sapiens
0.0114
-
dUMP pH 8.0, 25°C, recombinant enzyme Campylobacter jejuni
0.135
-
2'-deoxyuracil 5'-diphenyl phosphate pH 8.0, 25°C Homo sapiens
0.156
-
3-[5-(trityloxy)tetrahydrofuran-2-yl]pyridine-2,6(1H,3H)-dione pH 8.0, 25°C Homo sapiens
0.298
-
5'-adamantoyl-3'-methyl-2',3'-dideoxyuracil pH 8.0, 25°C Homo sapiens
0.35
-
5'-hexadecanoyl-3'-methyl-2',3'-dideoxyuracil pH 8.0, 25°C Homo sapiens
0.547
-
5'-adamantoyl-3'-methyl-2',3'-dideoxyuracil pH 8.0, 25°C, recombinant enzyme Homo sapiens
0.805
-
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2'-deoxyuracil pH 8.0, 25°C Homo sapiens
0.807
-
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-3'-methyl-2',3'-dideoxyuracil pH 8.0, 25°C Homo sapiens
0.908
-
2'-deoxy-5'-triphenylmethanyluracil pH 8.0, 25°C Homo sapiens