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Literature summary for 3.6.1.23 extracted from
- Kinetic and thermodynamic characterization of dUTP hydrolysis by Plasmodium falciparum dUTPase (2008), Biochim. Biophys. Acta, 1784, 1347-1355.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzyme is a potential drug target against malaria | Plasmodium falciparum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the recombinant enzyme | Plasmodium falciparum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-deoxyuridine | competitive inhibition | Plasmodium falciparum |  |
| alpha,beta-imido-dUTP | competitive inhibition | Plasmodium falciparum | |
| dUDP | competitive inhibition | Plasmodium falciparum | |
| dUMP | product, competitive inhibition | Plasmodium falciparum | |
| dUTP | - |
Plasmodium falciparum | |
| EDTA | causes a 105fold decrease and a 12fold increase of the kcat and Km values for dUTP hydrolysis, respectively, compared to the dUTP-Mg2+ complex | Plasmodium falciparum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten and ligand binding kinetics, overview. Thermodynamic parameters for the interaction between Plasmodium falciparum dUTPase and deoxyuridine derivatives at 25.2 °C and pH 7.0 | Plasmodium falciparum | |
| 0.0019 | - |
dUTP | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
| 0.0019 | - |
dUTP-Mg2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
| 0.0027 | - |
dUTP-Co2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
| 0.0137 | - |
dUTP-Mn2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can substitute the physiological cofactor Mg2+, however the kcat is significantly reduced compared to dUTP-Mg2+ | Plasmodium falciparum | |
| Mg2+ | dependent on, the energetic barrier of the reaction is 4fold higher when Mg2+ is depleted | Plasmodium falciparum | |
| Mn2+ | can substitute the physiological cofactor Mg2+, however the kcat is significantly reduced compared to dUTP-Mg2+ | Plasmodium falciparum | |
| NaCl | maximal activity at 0.1 M | Plasmodium falciparum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dUTP + H2O | Plasmodium falciparum | - |
dUMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme by gel filtration | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dUTP + H2O | - |
Plasmodium falciparum | dUMP + diphosphate | - |
? | |
| dUTP + H2O | dUTPase catalyzes the hydrolysis of the alpha,beta-diphosphate bond of dUTP | Plasmodium falciparum | dUMP + diphosphate | - |
? | |
| dUTP-Co2+ + H2O | - |
Plasmodium falciparum | dUMP-Co2+ + diphosphate | - |
? | |
| dUTP-Mg2+ + H2O | - |
Plasmodium falciparum | dUMP-Mg2+ + diphosphate | - |
? | |
| dUTP-Mn2+ + H2O | - |
Plasmodium falciparum | dUMP-Mn2+ + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | - |
Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| deoxyuridine 5'-triphosphate nucleotidohydrolase | - |
Plasmodium falciparum |
| dUTPase | - |
Plasmodium falciparum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Plasmodium falciparum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.4 | - |
dUTP | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
| 4.1 | - |
dUTP-Co2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
| 5.3 | - |
dUTP-Mn2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
| 13.3 | - |
dUTP-Mg2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Plasmodium falciparum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0718 | - |
dUTP-Mn2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
| 0.092 | - |
dUTP-Co2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
| 0.0955 | - |
dUTP-Mg2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
| 0.099 | - |
dUMP | pH 7.0, 25°C | Plasmodium falciparum | |
| 0.115 | - |
dUTP | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
| 0.121 | - |
dUTP | pH 7.0, 25°C, recombinant enzyme, without NaCl, presence of EDTA | Plasmodium falciparum | |
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