Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bis(acetylacetonato)oxovanadium(IV) | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli | |
Mn2+ | can replace for Mg2+ | Escherichia coli | |
VO2+ | results a higher activity than Mg2+ or Mn2+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Escherichia coli | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA | dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | - |
Escherichia coli | dUMP + diphosphate | - |
? | |
dUTP + H2O | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA | Escherichia coli | dUMP + diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
dUTP | pH 7.5, 25°C, in the absence of added metal ions | Escherichia coli | |
14.7 | - |
dUTP | pH 7.5, 25°C, in the presence of Mg2+ or Mn2+ | Escherichia coli | |
24.2 | - |
dUTP | pH 7.5, 25°C, in the presence of bis(acetylacetonato)oxovanadium(IV) | Escherichia coli | |
27.3 | - |
dUTP | pH 7.5, 25°C, in the presence of VO2+ or bis(acetylacetonato)oxovanadium(IV) | Escherichia coli |