Literature summary for 3.6.1.23 extracted from

Barabas, O.; Pongracz, V.; Kovari, J.; Wilmanns, M.; Vertessy, B.G.
Structural insights into the catalytic mechanism of phosphate ester hydrolysis by dUTPase (2004), J. Biol. Chem., 279, 42907-42915.

Search for more literature for 3.6.1.23

Cloned(Commentary)

Cloned (Comment)	Organism
native and mutant enzyme	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor diffusion method	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D90N	no effect on Km, strong decrease in catalytic activity, H-bonding significantly altered	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.45	-	dUTP	D90N mutant enzyme, pH 7.8, room temperature	Escherichia coli
0.5	-	dUTP	wild-type enzyme, pH 7.8, room temperature	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dUTP + H2O	Escherichia coli	removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA	dUMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P06968	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant proteins	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dUTP + H2O	-	Escherichia coli	dUMP + diphosphate	-	?
dUTP + H2O	removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA	Escherichia coli	dUMP + diphosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0003	-	dUTP	D90N mutant enzyme, pH 7.8, room temperature	Escherichia coli
11	-	dUTP	wild-type enzyme, pH 7.8, room temperature	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)