Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor-diffusion method | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | competitive feedback inhibition | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | lower affinity for dUTP than strict dUTPases | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
136000 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Methanocaldococcus jannaschii | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides the substrate thymidylate synthase and is therefore essential for the synthesis of dTTP | dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q57872 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Methanocaldococcus jannaschii |
Storage Stability | Organism |
---|---|
-70°C, 50 mM HEPES, pH 7.2, 150 mM NaCl, 2 mM dithiothreitol | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTTP + H2O | - |
Methanocaldococcus jannaschii | dTMP + diphosphate | - |
? | |
dUTP + H2O | - |
Methanocaldococcus jannaschii | dUMP + diphosphate | - |
? | |
dUTP + H2O | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides the substrate thymidylate synthase and is therefore essential for the synthesis of dTTP | Methanocaldococcus jannaschii | dUMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | two trimers assemble into a hexamer, crystal structure analysis | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
DCD-DUT | catalyzes the deamination of dCTP and the dephosphorylation of dUTP without releasing an intermediate | Methanocaldococcus jannaschii |