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Literature summary for 3.6.1.23 extracted from

  • Huffman, J.L.; Li, H.; White, R.H.; Tainer, J.A.
    Structural basis for recognition and catalysis by the bifunctional dCTP deaminase and dUTPase from Methanococcus jannaschii (2003), J. Mol. Biol., 331, 885-896.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor-diffusion method Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
dTTP competitive feedback inhibition Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information lower affinity for dUTP than strict dUTPases Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
136000
-
gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dUTP + H2O Methanocaldococcus jannaschii removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides the substrate thymidylate synthase and is therefore essential for the synthesis of dTTP dUMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57872
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Methanocaldococcus jannaschii

Storage Stability

Storage Stability Organism
-70°C, 50 mM HEPES, pH 7.2, 150 mM NaCl, 2 mM dithiothreitol Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTTP + H2O
-
Methanocaldococcus jannaschii dTMP + diphosphate
-
?
dUTP + H2O
-
Methanocaldococcus jannaschii dUMP + diphosphate
-
?
dUTP + H2O removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides the substrate thymidylate synthase and is therefore essential for the synthesis of dTTP Methanocaldococcus jannaschii dUMP + diphosphate
-
?

Subunits

Subunits Comment Organism
hexamer two trimers assemble into a hexamer, crystal structure analysis Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
DCD-DUT catalyzes the deamination of dCTP and the dephosphorylation of dUTP without releasing an intermediate Methanocaldococcus jannaschii