Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Aminouracil | 5 mM, 50% loss of activity | Allium cepa | |
6-aminouracil | 5 mM, 60% loss of activity | Allium cepa | |
EDTA | 1 mM, 65% loss of activity | Allium cepa | |
KCl | 0.4 mM, 29% loss of activity | Allium cepa | |
NEM | 12 mM, 14% loss of activity | Allium cepa | |
Uracil | 5 mM, 30% loss of activity | Allium cepa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
dUTP | - |
Allium cepa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+, Mn2+ or Zn2+ required for enzyme function | Allium cepa | |
Mn2+ | Mg2+, Mn2+ or Zn2+ required for enzyme function | Allium cepa | |
Zn2+ | Mg2+, Mn2+ or Zn2+ required for enzyme function | Allium cepa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
gel filtration | Allium cepa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Allium cepa | enzyme plays a key role in deoxyribonucleotide metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Allium cepa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | meristem cells, high levels of dUTPase in both resting and proliferating root meristem cells. Activity increase at the G1/S boundary, remains high throughout S phase, and shows almost undetectable levels during G1 and G2 | Allium cepa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCTP + H2O | no activity | Allium cepa | dCMP + diphosphate | - |
? | |
dTTP + H2O | no activity | Allium cepa | dTMP + diphosphate | - |
? | |
dUTP + H2O | - |
Allium cepa | dUMP + diphosphate | - |
? | |
dUTP + H2O | enzyme plays a key role in deoxyribonucleotide metabolism | Allium cepa | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
5 min, stable | Allium cepa |
45 | - |
5 min, 28% loss of activity | Allium cepa |
55 | - |
5 min, complete inactivation | Allium cepa |