Literature summary for 3.6.1.13 extracted from

Zha, M.; Zhong, C.; Peng, Y.; Hu, H.; Ding, J.
Crystal structures of human NUDT5 reveal insights into the structural basis of the substrate specificity (2006), J. Mol. Biol., 364, 1021-1033.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
in apo form, in complex with ADP-D-ribose, and in complex with AMP with bound Mg2+, hanging drop vapor diffusion method, using 160 mM sodium acetate (pH 5.5), and 25% (w/v) 2-methyl-2,4-pentanediol, or 300 mM di-ammonium hydrogen citrate, 6% (w/v) n-propanol and 15% (w/v) polyethylene glycol 3350, or 200 mM sodium acetate, 100 mM Tris-HCl (pH 8.0) and 30% (w/v) polyethylene glycol 4000	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ADP-D-ribose + H2O	Homo sapiens	-	AMP + D-ribose 5-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9UKK9	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Q-Sepharose column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP-D-ribose + H2O	-	Homo sapiens	AMP + D-ribose 5-phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
ADP-ribose pyrophosphatase	-	Homo sapiens
ADPRase	-	Homo sapiens
NUDT5	-	Homo sapiens

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Release 2023.1 (January 2023)