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Literature summary for 3.6.1.1 extracted from

  • Ginting, E.L.; Iwasaki, S.; Maeganeku, C.; Motoshima, H.; Watanabe, K.
    Expression, purification, and characterization of cold-adapted inorganic pyrophosphatase from psychrophilic Shewanella sp. AS-11 (2014), Prep. Biochem. Biotechnol., 44, 480-492.
    View publication on PubMed
Search for more literature for 3.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Shewanella sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme utilizes Mn2+ over Mg2+ for activity Shewanella sp.
Mn2+ the enzyme utilizes Mn2+ over Mg2+ for activity, Mn2+ activation increases the enzyme's activity at low temperatures Shewanella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, SDS-PAGE Shewanella sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Shewanella sp.
-
 2 phosphate
-
 ?
diphosphate + H2O Shewanella sp. AS-11
-
 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Shewanella sp.
-
-
-
Shewanella sp. AS-11
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and anion exchange column chromatography Shewanella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Shewanella sp. 2 phosphate
-
 ?
diphosphate + H2O
-
 Shewanella sp. AS-11 2 phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Shewanella sp.

Synonyms

Synonyms Comment Organism
inorganic pyrophosphatase
-
 Shewanella sp.
PPase
-
 Shewanella sp.