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Literature summary for 3.6.1.1 extracted from

  • Benini, S.; Wilson, K.
    Structure of the Mycobacterium tuberculosis soluble inorganic pyrophosphatase Rv3628 at pH 7.0 (2011), Acta Crystallogr. Sect. F, 67, 866-870.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant Rv3628, hanging drop vapour diffusion method, mixing of 0.001 ml protein in150 mM Na HEPES, pH 7.0, and 0.001 ml reservoir solution, containing 1.6 M NaKHPO4, pH 7.0, and equilibration against 1 ml reservoir solution, X-ray diffraction structure determination and analysis at 1.5 A resolution, modeling Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Mycobacterium tuberculosis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ determination of K+-binding sites Mycobacterium tuberculosis
Mg2+ required for catalysis Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Mycobacterium tuberculosis
-
2 phosphate
-
?
diphosphate + H2O Mycobacterium tuberculosis H37Rv
-
2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WI55
-
-
Mycobacterium tuberculosis H37Rv P9WI55
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
Mycobacterium tuberculosis 2 phosphate
-
?
diphosphate + H2O
-
Mycobacterium tuberculosis H37Rv 2 phosphate
-
?

Subunits

Subunits Comment Organism
More structure comparison of family I enzymes, overview Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
inorganic pyrophosphatase
-
Mycobacterium tuberculosis
Rv3628
-
Mycobacterium tuberculosis

General Information

General Information Comment Organism
evolution Mycobacterium tuberculosis and Mycobacterium leprae genomes include genes for the only two family I inorganic pyrophosphatases known to contain two histidines in the active site, structure comparison of family I enzymes, overview Mycobacterium tuberculosis
additional information His21 and His86 are not essential for diphosphate hydrolysis, but are responsible for a shift in the optimal pH for the reaction compared with the Escherichia coli enzyme Mycobacterium tuberculosis