Crystallization (Comment) | Organism |
---|---|
purified recombinant Rv3628, hanging drop vapour diffusion method, mixing of 0.001 ml protein in150 mM Na HEPES, pH 7.0, and 0.001 ml reservoir solution, containing 1.6 M NaKHPO4, pH 7.0, and equilibration against 1 ml reservoir solution, X-ray diffraction structure determination and analysis at 1.5 A resolution, modeling | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Mycobacterium tuberculosis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | determination of K+-binding sites | Mycobacterium tuberculosis | |
Mg2+ | required for catalysis | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Mycobacterium tuberculosis | - |
2 phosphate | - |
? | |
diphosphate + H2O | Mycobacterium tuberculosis H37Rv | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WI55 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WI55 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Mycobacterium tuberculosis | 2 phosphate | - |
? | |
diphosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure comparison of family I enzymes, overview | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
inorganic pyrophosphatase | - |
Mycobacterium tuberculosis |
Rv3628 | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
evolution | Mycobacterium tuberculosis and Mycobacterium leprae genomes include genes for the only two family I inorganic pyrophosphatases known to contain two histidines in the active site, structure comparison of family I enzymes, overview | Mycobacterium tuberculosis |
additional information | His21 and His86 are not essential for diphosphate hydrolysis, but are responsible for a shift in the optimal pH for the reaction compared with the Escherichia coli enzyme | Mycobacterium tuberculosis |