Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3)pLysS cells | Thermococcus onnurineus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0188 | - |
diphosphate | pH and temperature not specified in the publication | Thermococcus onnurineus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | about 15% activity in the presence of 50 mM Co2+ compared to 50 mM Mg2+ | Thermococcus onnurineus | |
Mg2+ | maximal activity in the presence of 0.2 mM Mg2+ | Thermococcus onnurineus | |
additional information | not affected by 50 mM Mn2+, Ni2+, Ca2+, Zn2+, and Cu2+ | Thermococcus onnurineus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Thermococcus onnurineus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus onnurineus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon metal affinity chromatography | Thermococcus onnurineus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-AMP + H2O | 4% activity compared to diphosphate | Thermococcus onnurineus | adenosine + phosphate | - |
? | |
ADP + H2O | 5% activity compared to diphosphate | Thermococcus onnurineus | AMP + phosphate | - |
? | |
ATP + H2O | 4% activity compared to diphosphate | Thermococcus onnurineus | ADP + phosphate | - |
? | |
diphosphate + H2O | 100% activity | Thermococcus onnurineus | phosphate + phosphate | - |
? | |
additional information | no activity with PAP, PAPS, 3'-CMP, fructose 1,6-bisphosphate, and D-myo-inositol 1-monophosphate | Thermococcus onnurineus | ? | - |
? | |
polyphosphate + H2O | less than 1% activity compared to diphosphate | Thermococcus onnurineus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Thermococcus onnurineus |
Synonyms | Comment | Organism |
---|---|---|
inorganic pyrophosphatase | - |
Thermococcus onnurineus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
diphosphate | pH and temperature not specified in the publication | Thermococcus onnurineus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | - |
Thermococcus onnurineus |