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Literature summary for 3.6.1.1 extracted from
- Some characteristics of cotton pyrophosphatase activation by magnesium (2006), Russ. J. Plant Physiol., 53, 48-53.
No PubMed abstract available
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can displace Mg2+ | Gossypium hirsutum |  |
| Mg2+ | activates by decreasing the value of the Michaelis-Menten constant, dependent on, highest activity at pH 7.4 and 5.0 mM, Mg2+ shifts the pH optimum, Mg2+ associates rather weakly with the pyrophosphatase active center and can be readily displaced by Ca2+ , overview | Gossypium hirsutum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gossypium hirsutum | - |
cv. 108-F | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from germinated seeds by acetone precipitation, gel ifltration, and anion exchange chromatography, 51.7fold | Gossypium hirsutum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | germinating, presence of Mg2+ ions in the germination medium markedly shortens time for the attainment of the diphosphatase maximum activity, overview | Gossypium hirsutum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 415 | - |
purified native enzyme | Gossypium hirsutum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pyrophosphatase | - |
Gossypium hirsutum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 8.6 | broad optimum, the pH optimum shifts with binding of Mg2+, overview | Gossypium hirsutum |
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Release 2023.1 (January 2023)
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