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Literature summary for 3.6.1.1 extracted from

  • Gomez-Garcia, M.R.; Losada, M.; Serrano, A.
    Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria (2007), FEBS J., 274, 3948-3959.
    View publication on PubMed
Search for more literature for 3.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene ppa, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity Anabaena sp.
gene ppa, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity, expression in Escherichia coli strain XL1-blue Synechocystis sp.
gene ppa, DNA and amino acid sequence determination and analysis,phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity Pseudanabaena sp.
gene ppa, phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity Blastochloris viridis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 diphosphate native enzyme Synechocystis sp.
0.0029
-
 diphosphate
-
 Pseudanabaena sp.
0.003
-
 diphosphate
-
 Anabaena sp.
0.003
-
 diphosphate recombinant enzyme Synechocystis sp.
0.0274
-
 diphosphate
-
 Blastochloris viridis

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Blastochloris viridis
-
-
soluble
-
 Synechocystis sp.
-
-
soluble
-
 Anabaena sp.
-
-
soluble
-
 Pseudanabaena sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates 25% Blastochloris viridis
Cu2+ activates Blastochloris viridis
Fe2+ activates 34% Blastochloris viridis
Mg2+ activates, Mg2+ is the highly preferred cofactor for family I sPPases Synechocystis sp.
Mg2+ activates, Mg2+ is the highly preferred cofactor for family I sPPases Anabaena sp.
Mg2+ activates, Mg2+ is the highly preferred cofactor for family I sPPases Pseudanabaena sp.
Mg2+ activates, Mg2+ is the preferred cofactor for family I sPPases Blastochloris viridis
Mn2+ activates Blastochloris viridis
additional information the enzyme is absolutely dependent on divalent cations Blastochloris viridis
additional information the enzyme is absolutely dependent on divalent cations, poor activity with Mn2+, Cu2+, Fe2+, and Co2+ Synechocystis sp.
additional information the enzyme is absolutely dependent on divalent cations, poor activity with Mn2+, Cu2+, Fe2+, Zn2+, and Co2+ Anabaena sp.
additional information the enzyme is absolutely dependent on divalent cations, poor activity with Mn2+, Cu2+, Fe2+, Zn2+, and Co2+ Pseudanabaena sp.
Zn2+ 13% of the activity with Mg2+ Synechocystis sp.
Zn2+ activates 40% Blastochloris viridis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 x * 20000, SDS-PAGE Pseudanabaena sp.
20000
-
 6 * 20000, SDS-PAGE Anabaena sp.
20000
-
 12 * 20000 Blastochloris viridis
110000
-
 gel filtration Synechocystis sp.
114000
-
 native PAGE Anabaena sp.
120000
-
 native PAGE Synechocystis sp.
240000
-
 native PAGE Blastochloris viridis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Blastochloris viridis an essential and ubiquitous metal-dependent enzyme 2 phosphate
-
 ?
diphosphate + H2O Synechocystis sp. an essential and ubiquitous metal-dependent enzyme 2 phosphate
-
 ?
diphosphate + H2O Anabaena sp. an essential and ubiquitous metal-dependent enzyme 2 phosphate
-
 ?
diphosphate + H2O Pseudanabaena sp. an essential and ubiquitous metal-dependent enzyme 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Anabaena sp. P80562 strain PCC 7120, gene ppa
-
Blastochloris viridis
-
 gene ppa
-
Pseudanabaena sp. P58733 strain PCC 6903, gene ppa
-
Synechocystis sp. P80507 strain PCC 6803, gene ppa
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme Anabaena sp.
native enzyme, and recombinant enzyme from Escherichia coli strain XL1-blue Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Blastochloris viridis 2 phosphate
-
 ?
diphosphate + H2O
-
 Synechocystis sp. 2 phosphate
-
 ?
diphosphate + H2O
-
 Anabaena sp. 2 phosphate
-
 ?
diphosphate + H2O
-
 Pseudanabaena sp. 2 phosphate
-
 ?
diphosphate + H2O an essential and ubiquitous metal-dependent enzyme Blastochloris viridis 2 phosphate
-
 ?
diphosphate + H2O an essential and ubiquitous metal-dependent enzyme Synechocystis sp. 2 phosphate
-
 ?
diphosphate + H2O an essential and ubiquitous metal-dependent enzyme Anabaena sp. 2 phosphate
-
 ?
diphosphate + H2O an essential and ubiquitous metal-dependent enzyme Pseudanabaena sp. 2 phosphate
-
 ?

Subunits

Subunits Comment Organism
dodecamer 12 * 20000 Blastochloris viridis
hexamer 6 * 20000, SDS-PAGE Anabaena sp.
hexamer 6 * 19 187, mass spectrometry Synechocystis sp.
oligomer x * 20000, SDS-PAGE Pseudanabaena sp.

Synonyms

Synonyms Comment Organism
family I inorganic pyrophosphatase
-
 Blastochloris viridis
family I inorganic pyrophosphatase
-
 Synechocystis sp.
family I inorganic pyrophosphatase
-
 Anabaena sp.
family I inorganic pyrophosphatase
-
 Pseudanabaena sp.
soluble inorganic pyrophosphatase
-
 Blastochloris viridis
soluble inorganic pyrophosphatase
-
 Synechocystis sp.
soluble inorganic pyrophosphatase
-
 Anabaena sp.
soluble inorganic pyrophosphatase
-
 Pseudanabaena sp.
sPPAse
-
 Blastochloris viridis
sPPAse
-
 Synechocystis sp.
sPPAse
-
 Anabaena sp.
sPPAse
-
 Pseudanabaena sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
800
-
 diphosphate recombinant enzyme Synechocystis sp.
850
-
 diphosphate
-
 Anabaena sp.
850
-
 diphosphate
-
 Pseudanabaena sp.
916
-
 diphosphate native enzyme Synechocystis sp.

pI Value

Organism Comment pI Value Maximum pI Value
Synechocystis sp. column chromatofocusing
-
 4.7