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Literature summary for 3.6.1.1 extracted from

  • Beknazarov, B.O.; Valikhanov, M.N.
    Properties of cotton inorganic pyrophosphatase (2007), Appl. Biochem. Microbiol., 43, 153-158.
No PubMed abstract available
Search for more literature for 3.6.1.1

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the purified enzyme on hydrophobic supports polycephamide and lysopolycephamide allows preservation of 24.2% and 17.3% of the starting catalytic activities with activity of imMobilized enzymes per gram support of 507 and 285 U/g, respectively Gossypium hirsutum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ activates at up to 1 mM, inhibits at higher concentrations, complete inhibition at 2 mM, overview Gossypium hirsutum
Co2+ activates at up to 1 mM, inhibits at higher concentrations, overview Gossypium hirsutum
fluoride 50% inhibition at 0.5 mM, 70-80% at 1.0 mM Gossypium hirsutum
Mn2+ activates at up to 1 mM, inhibits at higher concentrations, overview Gossypium hirsutum
Zn2+ activates at up to 1 mM, inhibits at higher concentrations, overview Gossypium hirsutum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates at up to 1 mM, inhibits at higher concentrations, complete inhibition at 2 mM, overview Gossypium hirsutum
Co2+ activates at up to 1 mM, inhibits at higher concentrations, overview Gossypium hirsutum
Mg2+ 7-8fold activation Gossypium hirsutum
Mn2+ activates at up to 1 mM, inhibits at higher concentrations, overview Gossypium hirsutum
Zn2+ highly activates at up to 1 mM, inhibits at higher concentrations, overview Gossypium hirsutum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 gel filtration Gossypium hirsutum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Gossypium hirsutum
-
 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Gossypium hirsutum
-
 var. 108-F
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from seedlings 30fold by acetone precipitation, gel filtration, and anion exchange chromatography Gossypium hirsutum

Source Tissue

Source Tissue Comment Organism Textmining
seedling the activity of inorganic diphosphatase reaches its maximum 1 day after the beginning of seed bud development, and begins to decrease after 2 days with 34% of its maximum value after 3 days of seed bud development Gossypium hirsutum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 0.0185 mMol/g of tissue after 2 days of seed bud development Gossypium hirsutum
240
-
 purified native enzyme Gossypium hirsutum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Gossypium hirsutum 2 phosphate
-
 ?
diphosphate + H2O the enzyme is very specific for diphosphate as substrate, substrate specificity, overview Gossypium hirsutum 2 phosphate
-
 ?

Synonyms

Synonyms Comment Organism
inorganic pyrophosphatase
-
 Gossypium hirsutum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55
-
 Gossypium hirsutum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 purified enzyme, 70-80% remaining activity Gossypium hirsutum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 pH optimum in absence of Mg2+ Gossypium hirsutum
8.6
-
 pH optimum in presence of Mg2+ Gossypium hirsutum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.42
-
 additional information pH 7.2, 37°C, fluoride inhibition kinetics Gossypium hirsutum