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Literature summary for 3.6.1.1 extracted from
- Three extremely thermostable proteins from Sulfolobuc and a reappraisal of the 'traffic rules' (1996), Biol. Chem., 377, 505-512.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Sulfolobus acidocaldarius |
General Stability
| General Stability | Organism |
|---|---|
| 5 mM Mg2+ stabilizes, the midpoint of thermal denaturation is increased to 98°C | Sulfolobus acidocaldarius |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Sulfolobus acidocaldarius | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19365 | - |
4 * 19365, calculated from sequence | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | P50308 | - |
- |
| Sulfolobus acidocaldarius DSM 639 | P50308 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Sulfolobus acidocaldarius | 2 phosphate | - |
? |  |
| diphosphate + H2O | - |
Sulfolobus acidocaldarius DSM 639 | 2 phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 19365, calculated from sequence | Sulfolobus acidocaldarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inorganic pyrophosphatase | - |
Sulfolobus acidocaldarius |
| PPase | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Sulfolobus acidocaldarius |
| 75 | - |
- |
Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
more than 24 h | Sulfolobus acidocaldarius |
| 80 | - |
half-time of inactivation is 10 h | Sulfolobus acidocaldarius |
| 85 | - |
24 h, 19% loss of activity | Sulfolobus acidocaldarius |
| 87 | - |
Tm-value | Sulfolobus acidocaldarius |
| 90 | - |
4 h, 60% loss of activity | Sulfolobus acidocaldarius |
| 95 | - |
4 h, 85% loss of activity | Sulfolobus acidocaldarius |
| 98 | - |
in the presence of 5 mM Mg2+ the midpoint of thermal denaturation is increased to 98°C | Sulfolobus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Sulfolobus acidocaldarius |
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Release 2023.1 (January 2023)
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