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Literature summary for 3.6.1.1 extracted from

  • Ohnishi, M.; Mimura, T.; Tsujimura, T.; Mitsuhashi, N.; Washitani-Nemoto, S.; Maeshima, M.; Martinoia, E.
    Inorganic phosphate uptake in intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status (2006), Planta, 225, 711-718.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuolar membrane
-
Catharanthus roseus 5774
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Catharanthus roseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Catharanthus roseus
-
2 phosphate
-
?
additional information Catharanthus roseus the enzyme is a V-type vacuolar H+-pump and responsible for phosphate uptake across the vacuolar membrane, mechanism of activation of phosphate uptake into the vacuole under low phosphate status, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially by vacuole membrane-enriched fraction preparation Catharanthus roseus

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Catharanthus roseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of H+-PPase measured as K+/Na+-dependent diphosphate hydrolysis activity, phosphate uptake of vacuoles isolated from high phosphate and low phosphate cells, activity profiles, cellular phosphate content, overview Catharanthus roseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
Catharanthus roseus 2 phosphate
-
?
additional information the enzyme is a V-type vacuolar H+-pump and responsible for phosphate uptake across the vacuolar membrane, mechanism of activation of phosphate uptake into the vacuole under low phosphate status, overview Catharanthus roseus ?
-
?

Synonyms

Synonyms Comment Organism
H+-PPase
-
Catharanthus roseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Catharanthus roseus