Cloned (Comment) | Organism |
---|---|
overexpression of soluble wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Streptococcus gordonii |
Protein Variants | Comment | Organism |
---|---|---|
G190A | site-directed mutagenesis, the mutant shows altered kinetic parameters and reduced activity compared to the wild-type enzyme | Streptococcus gordonii |
G190W | site-directed mutagenesis, the mutant shows altered kinetic parameters and reduced activity compared to the wild-type enzyme | Streptococcus gordonii |
additional information | construction of several deletion and insertion mutants, overview, mutants with altered interdomain region display 2-265fold decreased catalytic efficiency, overview | Streptococcus gordonii |
T191G | site-directed mutagenesis, the mutant shows altered kinetic parameters and reduced activity compared to the wild-type enzyme | Streptococcus gordonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and mutant enzymes | Streptococcus gordonii | |
0.062 | - |
diphosphate | pH 9.0, 25°C, 5 mM Mg2+, wild-type enzyme | Streptococcus gordonii | |
0.07 | - |
diphosphate | pH 9.0, 25°C, 5 mM Mg2+, mutant G190W | Streptococcus gordonii | |
0.081 | - |
diphosphate | pH 9.0, 25°C, 5 mM Mg2+, mutant T191G | Streptococcus gordonii | |
0.123 | - |
diphosphate | pH 9.0, 25°C, 5 mM Mg2+, mutant G190A | Streptococcus gordonii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Streptococcus gordonii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme requires divalent cations, 5 mM | Streptococcus gordonii | |
Mn2+ | the enzyme requires divalent cations | Streptococcus gordonii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is ellipsoidal with an axial ratio of 3.37, which increases in absence of divalent cation to 3.94, analytical ultracentrifugation | Streptococcus gordonii |
33407 | - |
2 * 33407, mass spectrometry | Streptococcus gordonii |
67000 | - |
native PAGE and analytical ultracentrifugation | Streptococcus gordonii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Streptococcus gordonii | - |
2 phosphate | - |
? | |
diphosphate + H2O | Streptococcus gordonii DL1 | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus gordonii | P95765 | - |
- |
Streptococcus gordonii DL1 | P95765 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography | Streptococcus gordonii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptococcus gordonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Streptococcus gordonii | 2 phosphate | - |
? | |
diphosphate + H2O | - |
Streptococcus gordonii DL1 | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33407, mass spectrometry | Streptococcus gordonii |
More | the free N- and C-terminal domains do not interact productively, when mixed together, the interdomain region has the function of a mechanical hinge, structure modelling | Streptococcus gordonii |
Synonyms | Comment | Organism |
---|---|---|
family II PPase | - |
Streptococcus gordonii |
PPase | - |
Streptococcus gordonii |
soluble inorganic pyrophosphatase | - |
Streptococcus gordonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptococcus gordonii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2130 | - |
diphosphate | pH 9.0, 25°C, 5 mM Mg2+, mutant T191G | Streptococcus gordonii | |
3270 | - |
diphosphate | pH 9.0, 25°C, 5 mM Mg2+, mutant G190A | Streptococcus gordonii | |
3460 | - |
diphosphate | pH 9.0, 25°C, 5 mM Mg2+, mutant G190W | Streptococcus gordonii | |
6290 | - |
diphosphate | pH 9.0, 25°C, 5 mM Mg2+, wild-type enzyme | Streptococcus gordonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Streptococcus gordonii |