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Literature summary for 3.6.1.1 extracted from

  • Ilias, M.; Young, T.W.
    Streptococcus gordonii soluble inorganic pyrophosphatase: An important role for the interdomain region in enzyme activity (2006), Biochim. Biophys. Acta, 1764, 1299-1306.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of soluble wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Streptococcus gordonii

Protein Variants

Protein Variants Comment Organism
G190A site-directed mutagenesis, the mutant shows altered kinetic parameters and reduced activity compared to the wild-type enzyme Streptococcus gordonii
G190W site-directed mutagenesis, the mutant shows altered kinetic parameters and reduced activity compared to the wild-type enzyme Streptococcus gordonii
additional information construction of several deletion and insertion mutants, overview, mutants with altered interdomain region display 2-265fold decreased catalytic efficiency, overview Streptococcus gordonii
T191G site-directed mutagenesis, the mutant shows altered kinetic parameters and reduced activity compared to the wild-type enzyme Streptococcus gordonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of wild-type and mutant enzymes Streptococcus gordonii
0.062
-
diphosphate pH 9.0, 25°C, 5 mM Mg2+, wild-type enzyme Streptococcus gordonii
0.07
-
diphosphate pH 9.0, 25°C, 5 mM Mg2+, mutant G190W Streptococcus gordonii
0.081
-
diphosphate pH 9.0, 25°C, 5 mM Mg2+, mutant T191G Streptococcus gordonii
0.123
-
diphosphate pH 9.0, 25°C, 5 mM Mg2+, mutant G190A Streptococcus gordonii

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Streptococcus gordonii
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme requires divalent cations, 5 mM Streptococcus gordonii
Mn2+ the enzyme requires divalent cations Streptococcus gordonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
the enzyme is ellipsoidal with an axial ratio of 3.37, which increases in absence of divalent cation to 3.94, analytical ultracentrifugation Streptococcus gordonii
33407
-
2 * 33407, mass spectrometry Streptococcus gordonii
67000
-
native PAGE and analytical ultracentrifugation Streptococcus gordonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Streptococcus gordonii
-
2 phosphate
-
?
diphosphate + H2O Streptococcus gordonii DL1
-
2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus gordonii P95765
-
-
Streptococcus gordonii DL1 P95765
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography Streptococcus gordonii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Streptococcus gordonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
Streptococcus gordonii 2 phosphate
-
?
diphosphate + H2O
-
Streptococcus gordonii DL1 2 phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 33407, mass spectrometry Streptococcus gordonii
More the free N- and C-terminal domains do not interact productively, when mixed together, the interdomain region has the function of a mechanical hinge, structure modelling Streptococcus gordonii

Synonyms

Synonyms Comment Organism
family II PPase
-
Streptococcus gordonii
PPase
-
Streptococcus gordonii
soluble inorganic pyrophosphatase
-
Streptococcus gordonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Streptococcus gordonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2130
-
diphosphate pH 9.0, 25°C, 5 mM Mg2+, mutant T191G Streptococcus gordonii
3270
-
diphosphate pH 9.0, 25°C, 5 mM Mg2+, mutant G190A Streptococcus gordonii
3460
-
diphosphate pH 9.0, 25°C, 5 mM Mg2+, mutant G190W Streptococcus gordonii
6290
-
diphosphate pH 9.0, 25°C, 5 mM Mg2+, wild-type enzyme Streptococcus gordonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Streptococcus gordonii