Cloned (Comment) | Organism |
---|---|
gene ppa1, isozyme PPase 1, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli | Arabidopsis thaliana |
gene ppa1, isozyme sPPase-I, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Chlamydomonas reinhardtii |
gene ppa2, isozyme sPPase-II, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Chlamydomonas reinhardtii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
chloroplast | isozyme sPPase-I | Chlamydomonas reinhardtii | 9507 | - |
mitochondrion | isozyme sPPase-II | Chlamydomonas reinhardtii | 5739 | - |
additional information | isozyme subcellular localization study | Chlamydomonas reinhardtii | - |
- |
soluble | isozyme sPPase-I | Chlamydomonas reinhardtii | - |
- |
soluble | isozyme sPPase-II | Chlamydomonas reinhardtii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | can partially, upt to 30%, substitute for Mg2+ | Chlamydomonas reinhardtii | |
Fe2+ | can partially, upt to 30%, substitute for Mg2+ | Chlamydomonas reinhardtii | |
Mg2+ | required, best divalent cation | Chlamydomonas reinhardtii | |
Mn2+ | can partially, upt to 30%, substitute for Mg2+ | Chlamydomonas reinhardtii | |
Zn2+ | can partially, upt to 30%, substitute for Mg2+ | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
1 * 24000, isozyme sPPase-II, SDS-PAGE, 1 * 24350, isozyme sPPase-II, mass spectrometry | Chlamydomonas reinhardtii |
24350 | - |
1 * 24000, isozyme sPPase-II, SDS-PAGE, 1 * 24350, isozyme sPPase-II, mass spectrometry | Chlamydomonas reinhardtii |
28000 | 32000 | gel filtration and native PAGE | Chlamydomonas reinhardtii |
29850 | - |
1 * 30000, isozyme sPPase-I, SDS-PAGE, 1 * 29850, isozyme sPPase-I, mass spectrometry | Chlamydomonas reinhardtii |
30000 | - |
1 * 30000, isozyme sPPase-I, SDS-PAGE, 1 * 29850, isozyme sPPase-I, mass spectrometry | Chlamydomonas reinhardtii |
33000 | 37000 | gel filtration and native PAGE | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Chlamydomonas reinhardtii | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LXC9 | isozyme PPA1, chloroplast precursor; isozyme PPA1 | - |
Chlamydomonas reinhardtii | Q93Y52 | ppa1, chloroplast precursor; soluble inorganic pyrophosphatase isozyme I, plastidial isozyme sPPase-I, gene ppa1, is the major isozyme | - |
Chlamydomonas reinhardtii | Q949J1 | ppa2; soluble inorganic pyrophosphatase isozyme II, minor isozyme | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the chloroplast isozyme I contains a precursor sequence, the recombinant enzyme is processed in bacterial cells | Chlamydomonas reinhardtii |
Purification (Comment) | Organism |
---|---|
native isozyme sPPase-I by streptomycin and ammonium sulfate fractionation, dialysis, anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography and gel filtration | Chlamydomonas reinhardtii |
native isozyme sPPase-II by streptomycin and ammonium sulfate fractionation, dialysis, anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography and gel filtration | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Chlamydomonas reinhardtii | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 24000, isozyme sPPase-II, SDS-PAGE, 1 * 24350, isozyme sPPase-II, mass spectrometry | Chlamydomonas reinhardtii |
monomer | 1 * 30000, isozyme sPPase-I, SDS-PAGE, 1 * 29850, isozyme sPPase-I, mass spectrometry | Chlamydomonas reinhardtii |
More | peptide mass fingerprint analysis of isozyme sPPase-I | Chlamydomonas reinhardtii |
More | peptide mass fingerprint analysis of isozyme sPPase-II | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
chloroplast inorganic pyrophosphatase 1 | - |
Arabidopsis thaliana |
More | the enzyme is a member of the sPPase family I | Chlamydomonas reinhardtii |
PPA1 | - |
Arabidopsis thaliana |
PPase 1 | - |
Chlamydomonas reinhardtii |
PPase 1 | - |
Arabidopsis thaliana |
PPase 2 | - |
Chlamydomonas reinhardtii |
pyrophosphate phospho-hydrolase 1 | - |
Arabidopsis thaliana |
soluble inorganic pyrophosphatase | - |
Chlamydomonas reinhardtii |
soluble inorganic pyrophosphatase 2 | - |
Chlamydomonas reinhardtii |
sPPAse | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Chlamydomonas reinhardtii |