Literature summary for 3.6.1.1 extracted from

Wu, C.A.; Lokanath, N.K.; Kim, D.Y.; Park, H.J.; Hwang, H.Y.; Kim, S.T.; Suh, S.W.; Kim, K.K.
Structure of inorganic pyrophosphatase from Helicobacter pylori (2005), Acta Crystallogr. Sect. D, D61, 1459-1464.

Search for more literature for 3.6.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
gene Hp0620, structure-based phylogenetic tree of diphosphatases, expression of His-tagged enzyme in Escherichia coli strain B834(DE3) resulting in a selenomethionine-enzyme	Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged selenomethionine-enzyme, hanging drop vapour diffusion method, 14 mg/ml protein in 50 mM Tris-HCl, pH 7.0, 0.1 M NaCl, 1 mM 2-mercaptoethanol, and 10 mM MgCl2, against a reservoir solution containing 36% v/v methylpentanediol, 0.2 M MgCl2, and 0.1 M imidazole, pH 8.0, X-ray diffraction structure determination and analysis at 2.6 A resolution	Helicobacter pylori

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Helicobacter pylori	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required, best divalent cation, binding site structure	Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
19272	-	6 * 19272, amino acid sequence calculation, structure-based phylogenetic tree of diphosphatases, overview	Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
diphosphate + H2O	Helicobacter pylori	-	2 phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Helicobacter pylori	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged selenomethionine-enzyme from Escherichia coli strain B834(DE3) by metal affinity and anion exchange chromatography, and gel filtration	Helicobacter pylori

Reaction

Reaction	Comment	Organism	Reaction ID
diphosphate + H2O = 2 phosphate	catalytic mechanism involving residues Lys28, Tyr140, Glu20, Asp64, Asp69, Asp96, and Asp101, active site structure	Helicobacter pylori	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diphosphate + H2O	-	Helicobacter pylori	2 phosphate	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 * 19272, amino acid sequence calculation, structure-based phylogenetic tree of diphosphatases, overview	Helicobacter pylori

Synonyms

Synonyms	Comment	Organism
inorganic pyrophosphatase	-	Helicobacter pylori
PPase	-	Helicobacter pylori

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Release 2023.1 (January 2023)