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Literature summary for 3.6.1.1 extracted from

  • Gomez-Garcia, M.R.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.; Serrano, A.
    A novel calcium-dependent soluble inorganic pyrophosphatase from the trypanosomatid Leishmania major (2004), FEBS Lett., 560, 158-166.
    View publication on PubMed
Search for more literature for 3.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
 diphosphate pH 7.5, in the presence of Ca2+ Leishmania major
0.37
-
 diphosphate pH 7.5, in the presence of Mg2+ Leishmania major

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion soluble Leishmania major 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Leishmania major
Mg2+ much less effective than Ca2+, 20% of the activity with Ca2+ Leishmania major
additional information other metal ions can partially replace Ca+, activation decreases in the order Ca2+> Cu2+> Mn2+> Fe2+> Mg2+> Zn2+> Co2+ Leishmania major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28900
-
 calculated from predicted amino acid sequence Leishmania major
30000
-
 SDS-PAGE Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Leishmania major important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?
diphosphate + H2O Leishmania major 252 important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania major Q6UQ31
-
-
Leishmania major 252 Q6UQ31
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Leishmania major

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
154.3
-
 purified enzyme, pH 7.5 Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Leishmania major 2 phosphate
-
 ?
diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Leishmania major 2 phosphate
-
 ?
diphosphate + H2O
-
 Leishmania major 252 2 phosphate
-
 ?
diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Leishmania major 252 2 phosphate
-
 ?
additional information polyphosphates and ATP are not hydrolyzed Leishmania major ?
-
 ?
additional information polyphosphates and ATP are not hydrolyzed Leishmania major 252 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
369
-
 diphosphate pH 7.5, in the presence of Mg2+ Leishmania major
3693
-
 diphosphate pH 7.5, in the presence of Ca2+ Leishmania major

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Leishmania major

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Leishmania major

pI Value

Organism Comment pI Value Maximum pI Value
Leishmania major calculated from predicted amino acid sequence
-
 6