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Literature summary for 3.6.1.1 extracted from

  • Vainonen, Y.P.; Vorobyeva, N.N.; Kurilova, S.A.; Nazarova, T.I.; Rodina, E.V.; Avaeva, S.M.
    Active dimeric form of inorganic pyrophosphatase from Escherichia coli (2003), Biochemistry, 68, 1195-1199.
    View publication on PubMed
Search for more literature for 3.6.1.1

Activating Compound

Activating Compound Comment Organism Structure
methylenebisphosphonate activates the hexameric but not the dimeric form of the enzyme at pH 6 Escherichia coli

Protein Variants

Protein Variants Comment Organism
E145Q easy formation of dimers with 5% of activity of the corresponding hexamer, 5fold increase of Kd for Mg2+ at site 2, no inhibition with high concentrations of Mg2+ Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 diphosphate wild type hexamer, pH 7.5 Escherichia coli
0.8
-
 diphosphate wild type dimer, pH 7.5 Escherichia coli
1.3
-
 diphosphate E145Q hexamer, pH 7.5 Escherichia coli
1.8
-
 diphosphate E145Q dimer, pH 7.5 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, inhibitory for the native hexamer above 5 mM, no inhibition of the dimeric form Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Escherichia coli important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Escherichia coli 2 phosphate
-
 ?
diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Escherichia coli 2 phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer an active dimer can be obtained using 20% isopropanol from the native hexamer Escherichia coli
hexamer native form, composed of two trimers Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.7
-
 diphosphate E145Q mutant dimer, pH 7.5 Escherichia coli
76
-
 diphosphate wild type dimer, pH 7.5 Escherichia coli
83
-
 diphosphate E145Q mutant hexamer, pH 7.5 Escherichia coli
390
-
 diphosphate wild type hexamer, pH 7.5 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
 Escherichia coli